Fig. 5.

SMaRT genes are differentially translated in polarized macrophages. a) Overview of the experimental design. PMA-treated human THP-1 cell lines (M0) are polarized to M1 (with LPS and IFNγ) or M2 (with IL4), followed by multiplexed mass spectrometry at indicated time points. The fraction of the global macrophage transcriptome (from the pooled 197 macrophage datasets) that is represented in the global macrophage proteome is subsequently assessed for induction (or not) of proteins that are translated by various gene signatures. b) Selectivity of induction of proteins upon LPS and IFNγ (top) or IL4 (bottom) stimulation at various timepoints was assessed across different signatures using z-test of proportions and −log (10) p values are displayed as heatmaps. c and d) z normalized Log of intensities of proteins (Supplemental Information 3) translated at different time points by genes in C#13 (c) and C#14 + 3 (d) is displayed as heatmaps.