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. 1983 May;67(5):286–291. doi: 10.1136/bjo.67.5.286

Quantitation of angiogenesis factor in bovine retina and tumour extracts by means of radioimmunoassay.

S Shahabuddin, S Kumar
PMCID: PMC1040044  PMID: 6838799

Abstract

Using an antiserum raised against tumour angiogenesis factor (TAF) we have developed a radioimmunoassay for retina and tumour angiogenesis factor(s). This antiserum was previously shown to bind to both human and animal tumour extracts and to inhibit the angiogenesis induced by TAF in vivo. TAF from rat Walker tumour was used for iodination by the chloramine-T method. An excess of 125I-labelled TAF was incubated with TAF antibody in the absence (maximum binding) and presence (inhibition of maximum binding) of unlabelled tissue extract. A double antibody technique was used to separate free and bound TAF. Unlabelled human Wilms tumour TAF was used as a standard. The extent of inhibition of 125I-TAF-anti-TAF binding provided a measure of TAF in tissue extracts examined. Extracts of normal bovine retina, cornea, lung, aorta, lymph nodes, iris, vitreous humour, and human tumours and normal human pituitary and liver were assayed. Only bovine retina and human tumours were found to contain angiogenesis factor. These findings, together with our earlier results, suggest that angiogenesis factor from both bovine retina and human tumours induce angiogenesis in vivo and possess common antigenic determinants. The presence of angiogenesis factor in healthy retina and its relationship to neovascularisation in clinical conditions is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brem S., Brem H., Folkman J., Finkelstein D., Patz A. Prolonged tumor dormancy by prevention of neovascularization in the vitreous. Cancer Res. 1976 Aug;36(8):2807–2812. [PubMed] [Google Scholar]
  2. Brown R. A., Weiss J. B., Tomlinson I. W., Phillips P., Kumar S. Angiogenic factor from synovial fluid resembling that from tumours. Lancet. 1980 Mar 29;1(8170):682–685. [PubMed] [Google Scholar]
  3. Cassel G. H. A clinical perspective on retinal neovascularization research. Ann Ophthalmol. 1982 Aug;14(8):713–716. [PubMed] [Google Scholar]
  4. Chen C. H., Chen S. C. Angiogenic activity of vitreous and retinal extract. Invest Ophthalmol Vis Sci. 1980 Jun;19(6):596–602. [PubMed] [Google Scholar]
  5. D'Amore P. A., Glaser B. M., Brunson S. K., Fenselau A. H. Angiogenic activity from bovine retina: partial purification and characterization. Proc Natl Acad Sci U S A. 1981 May;78(5):3068–3072. doi: 10.1073/pnas.78.5.3068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Federman J. L., Brown G. C., Felberg N. T., Felton S. M. Experimental ocular angiogenesis. Am J Ophthalmol. 1980 Feb;89(2):231–237. doi: 10.1016/0002-9394(80)90116-6. [DOI] [PubMed] [Google Scholar]
  7. Felton S. M., Brown G. C., Felberg N. T., Federman J. L. Vitreous inhibition of tumor neovascularization. Arch Ophthalmol. 1979 Sep;97(9):1710–1713. doi: 10.1001/archopht.1979.01020020278019. [DOI] [PubMed] [Google Scholar]
  8. Folkman J., Merler E., Abernathy C., Williams G. Isolation of a tumor factor responsible for angiogenesis. J Exp Med. 1971 Feb 1;133(2):275–288. doi: 10.1084/jem.133.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glaser B. M., D'Amore P. A., Michels R. G., Brunson S. K., Fenselau A. H., Rice T., Patz A. The demonstration of angiogenic activity from ocular tissues. Preliminary report. Ophthalmology. 1980 May;87(5):440–446. doi: 10.1016/s0161-6420(80)35224-x. [DOI] [PubMed] [Google Scholar]
  10. Glaser B. M., D'Amore P. A., Michels R. G., Patz A., Fenselau A. Demonstration of vasoproliferative activity from mammalian retina. J Cell Biol. 1980 Feb;84(2):298–304. doi: 10.1083/jcb.84.2.298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  12. Henkind P. Ocular neovascularization. The Krill memorial lecture. Am J Ophthalmol. 1978 Mar;85(3):287–301. [PubMed] [Google Scholar]
  13. Kissun R. D., Garner A. Vasoformative properties of normal and hypoxic retinal tissue. Br J Ophthalmol. 1977 Jun;61(6):394–398. doi: 10.1136/bjo.61.6.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kissun R. D., Hill C. R., Garner A., Phillips P., Kumar S., Weiss J. B. A low-molecular-weight angiogenic factor in cat retina. Br J Ophthalmol. 1982 Mar;66(3):165–169. doi: 10.1136/bjo.66.3.165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Phillips P., Kumar S. Tumour angiogenesis factor (TAF) and its neutralisation by a xenogeneic antiserum. Int J Cancer. 1979 Jan 15;23(1):82–88. doi: 10.1002/ijc.2910230115. [DOI] [PubMed] [Google Scholar]
  17. Preis I., Langer R., Brem H., Folkman J. Inhibition of neovascularization by an extract derived from vitreous. Am J Ophthalmol. 1977 Sep;84(3):323–328. doi: 10.1016/0002-9394(77)90672-9. [DOI] [PubMed] [Google Scholar]
  18. Schor A. M., Kumar S., Phillips P. J. Quantitation of extracts containing tumour angiogenesis factor (TAF) by radioimmunometric and radioimmunoassays. Int J Cancer. 1980 Jun 15;25(6):773–779. doi: 10.1002/ijc.2910250613. [DOI] [PubMed] [Google Scholar]
  19. YALOW R. S., BERSON S. A. Immunoassay of endogenous plasma insulin in man. J Clin Invest. 1960 Jul;39:1157–1175. doi: 10.1172/JCI104130. [DOI] [PMC free article] [PubMed] [Google Scholar]

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