Skip to main content
PMC home page
The British Journal of Ophthalmology logoLink to The British Journal of Ophthalmology
. 1983 Dec;67(12):804–807. doi: 10.1136/bjo.67.12.804

Effect of hypothermic perfusion on corneal endothelial morphology.

M J Mannis, R B Miller, E C Carlson, D Hinds, D R May
PMCID: PMC1040208  PMID: 6671094

Abstract

The effect of moderate in-vivo hypothermic perfusion on corneal endothelial integrity was studied in the cat. Eleven cats underwent in-vivo anterior chamber perfusion for 30 minutes with either normothermic (23 degrees C) or hypothermic (5 degrees C) perfusate. Corneas were then evaluated clinically (biomicroscopy), functionally (vital staining), and morphologically (scanning electron microscopy) for changes attributable to hypothermic perfusion. All 3 modes of evaluation suggested no difference in corneal endothelial integrity under the 2 experimental perfusion conditions. At the clinical and scanning electron microscope levels hypothermic perfusion does not show any effects on the corneal endothelium. Regional hypothermia is of theoretical and potential utility in procedures involving prolonged intraocular perfusion.

Full text

PDF
804

Images in this article

805Image
on p.805

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binder P. S., Sternberg H., Wickman M. G., Worthen D. M. Corneal endothelial damage associated with phacoemulsification. Am J Ophthalmol. 1976 Jul;82(1):48–54. doi: 10.1016/0002-9394(76)90663-2. [DOI] [PubMed] [Google Scholar]
  2. Bricolo A., Ore G. D., Da Pian R., Faccioli F. Local cooling in spinal cord injury. Surg Neurol. 1976 Aug;6(2):101–106. [PubMed] [Google Scholar]
  3. Edelhauser H. F., Van Horn D. L., Hyndiuk R. A., Schultz R. O. Intraocular irrigating solutions. Their effect on the corneal endothelium. Arch Ophthalmol. 1975 Aug;93(8):648–657. doi: 10.1001/archopht.1975.01010020614011. [DOI] [PubMed] [Google Scholar]
  4. Edelhauser H. F., Van Horn D. L., Schultz R. O., Hyndiuk R. A. Comparative toxicity of intraocular irrigating solutions on the corneal endothelium. Am J Ophthalmol. 1976 Apr;81(4):473–481. doi: 10.1016/0002-9394(76)90304-4. [DOI] [PubMed] [Google Scholar]
  5. Engedal H., Skagseth E., Saetersdal T. S., Myklebust R. Cardiac hypothermia evaluated by ultrastructural studies in man. J Thorac Cardiovasc Surg. 1978 Apr;75(4):548–554. [PubMed] [Google Scholar]
  6. Farcon E. M., Morales P., al-Askari S. In vivo hypothermic perfusion during renal surgery. Urology. 1974 Apr;3(4):414–420. doi: 10.1016/s0090-4295(74)80152-4. [DOI] [PubMed] [Google Scholar]
  7. Fatt I., Forester J. F. Errors in eye tissue temperature measurements when using a metallic probe. Exp Eye Res. 1972 Nov;14(3):270–276. doi: 10.1016/0014-4835(72)90013-9. [DOI] [PubMed] [Google Scholar]
  8. Fisk R. L., Gelfand E. T., Callaghan J. C. Hypothermic coronary perfusion for intraoperative cardioplegia. Ann Thorac Surg. 1977 Jan;23:58–61. doi: 10.1016/s0003-4975(10)64070-2. [DOI] [PubMed] [Google Scholar]
  9. Freeman R. D., Fatt I. Environmental influences on ocular temperature. Invest Ophthalmol. 1973 Aug;12(8):596–602. [PubMed] [Google Scholar]
  10. Green K., Hull D. S., Vaughn E. D., Malizia A. A., Jr, Bowman K. Rabbit endothelial response to ophthalmic preservatives. Arch Ophthalmol. 1977 Dec;95(12):2218–2221. doi: 10.1001/archopht.1977.04450120124018. [DOI] [PubMed] [Google Scholar]
  11. HARRIS J. E., NORDQUIST L. T. The hydration of the cornea. I. The transport of water from the cornea. Am J Ophthalmol. 1955 Nov;40(5 Pt 2):100–110. doi: 10.1016/0002-9394(55)91842-0. [DOI] [PubMed] [Google Scholar]
  12. McGill C., Taylor B., Acland R., Flint L. Effects of cooling and intraluminal antiseptics on ischemia in small bowel and colon. Surg Forum. 1977;28:424–425. [PubMed] [Google Scholar]
  13. Polack F. M., Sugar A. The phacoemulsification procedure. II. Corneal endothelial changes. Invest Ophthalmol. 1976 Jun;15(6):458–469. [PubMed] [Google Scholar]
  14. Rosenbluth R. F., Fatt I. Temperature measurements in the eye. Exp Eye Res. 1977 Oct;25(4):325–341. doi: 10.1016/0014-4835(77)90100-2. [DOI] [PubMed] [Google Scholar]
  15. Spence D. J., Peyman G. A. A new technique for the vital staining of the corneal endothelium. Invest Ophthalmol. 1976 Dec;15(12):1000–1002. [PubMed] [Google Scholar]
  16. Swan H. Clinical hypothermia: a lady with a past and some promise for the future. Surgery. 1973 May;73(5):736–758. [PubMed] [Google Scholar]
  17. Van Horn D. L., Edelhauser H. F., DeBruin J. Functional and ultrastructural changes in cryopreserved corneas. Arch Ophthalmol. 1973 Oct;90(4):312–318. doi: 10.1001/archopht.1973.01000050314016. [DOI] [PubMed] [Google Scholar]
  18. Van Horn D. L., Edelhauser H. F., Prodanovich G., Eiferman R., Pederson H. F. Effect of the ophthalmic preservative thimerosal on rabbit and human corneal endothelium. Invest Ophthalmol Vis Sci. 1977 Apr;16(4):273–280. [PubMed] [Google Scholar]
  19. Van Horn D. L., Hanna C., Schultz R. O. Corneal cryopreservation. II. Ultrastructural and viability changes. Arch Ophthalmol. 1970 Nov;84(5):655–667. doi: 10.1001/archopht.1970.00990040657020. [DOI] [PubMed] [Google Scholar]
  20. Van Horn D. L., Sendele D. D., Seideman S., Buco P. J. Regenerative capacity of the corneal endothelium in rabbit and cat. Invest Ophthalmol Vis Sci. 1977 Jul;16(7):597–613. [PubMed] [Google Scholar]
  21. WILSON G. S. Clinical experience in renal hypothermia. J Urol. 1963 May;89:666–669. doi: 10.1016/S0022-5347(17)64616-0. [DOI] [PubMed] [Google Scholar]

Articles from The British Journal of Ophthalmology are provided here courtesy of BMJ Publishing Group

RESOURCES