Figure 5. TGF-β epigenetically and transcriptionally alters memory CD8 T cell chemotaxis.

(A) Selected DE genes from RNA-seq and indicated statistical significance. (B) Chromatin accessibility of selected genes from ATAC-seq. (C) Frequency of low and high CCR8 expression by flow cytometry in OT-I T_mem across 24h stimulation conditions with TGF-β addition at indicated timepoints. (D) Frequency of CCR8-high expression plotted individually and indicated statistical significance. Stimulations in (C and D) are from the same experiments shown in Fig. 3A. Indicated statistical significances were calculated using one-way Anova with Tukey’s multiple comparison test. In (A and B) RNA- and ATAC-seq data depicted are from n = 7 animals. In (C) and (D) data depicted are from n = 3. Data shown are from 2 independent experiments.