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. 1984 Jun;68(6):405–408. doi: 10.1136/bjo.68.6.405

Rapid culture test for adenovirus isolation.

S Darougar, P Walpita, U Thaker, N Viswalingam, M S Wishart
PMCID: PMC1040363  PMID: 6326797

Abstract

A rapid culture test has been developed and evaluated for the detection of adenovirus in ocular infections. The test requires only two days' incubation of the inoculated cell monolayers on coverslips in flat bottomed tubes followed by detection of adenovirus inclusions using immunofluorescence staining method. The sensitivity of the rapid test is found to be comparable with that of the conventional tissue culture test which depends on the development of a cytopathic effect requiring 2-21 days' incubation (mean 9 X 4, SD 4 X 9).

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Selected References

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  1. DAVIDSON S. I. EPIDEMIC KERATO-CONJUNCTIVITIS. REPORT OF AN OUTBREAK WHICH RESULTED IN WARD CROSS-INFECTION. Br J Ophthalmol. 1964 Nov;48:573–580. doi: 10.1136/bjo.48.11.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gardner P. S., McQuillin J., Black M. M., Richardson J. Rapid diagnosis of herpesvirus hominis infections in superficial lesions by immunofluorescent antibody technics. Br Med J. 1968 Oct 12;4(5623):89–92. doi: 10.1136/bmj.4.5623.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KENDALL E. J., RIDDLE R. W., TUCK H. A., RODAN K. S., ANDREWS B. E., MCDONALD J. C. Pharyngo-conjunctival fever; school outbreaks in England during the summer of 1955 associated with adenovirus types 3, 7, and 14. Br Med J. 1957 Jul 20;2(5037):131–136. doi: 10.1136/bmj.2.5037.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. McSwiggan D. A., Darougar S., Rahman A. F., Gibson J. A. Comparison of the sensitivity of human embryo kidney cells, HeLa cells, and WI38 cells for the primary isolation of viruses from the eye. J Clin Pathol. 1975 May;28(5):410–413. doi: 10.1136/jcp.28.5.410. [DOI] [PMC free article] [PubMed] [Google Scholar]

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