Abstract
A rapid and sensitive culture test has been developed for detecting herpes simplex virus (HSV) in ocular infections. The virus is cultured by inoculation and centrifugation of cell monolayers grown on coverslips and the inclusions detected by an indirect immunofluorescence technique. This rapid test takes only two days to complete. By comparison, in our hands the conventional culture test, which depends on the development of cytopathic effect, took between 1 and 20 days with a mean of 4.7 days. Of the 1638 ocular clinical specimens inoculated in parallel by the two methods a total of 188 were positive for HSV. The virus was detected from 184 (97.8%) specimens by the rapid test and from 144 (76.6%) by the conventional test (McNemar's test, U = 5.76, p less than 0.001).
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- Darougar S., Gibson J. A., Thaker U. Effect of centrifugation on herpes simplex virus isolation. J Med Virol. 1981;8(4):231–235. doi: 10.1002/jmv.1890080403. [DOI] [PubMed] [Google Scholar]
- Darougar S., Hunter P. A., Viswalingam M., Gibson J. A., Jones B. R. Acute follicular conjunctivitis and keratoconjunctivitis due to herpes simplex virus in London. Br J Ophthalmol. 1978 Dec;62(12):843–849. doi: 10.1136/bjo.62.12.843. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Darougar S., Wishart M. S., Viswalingam N. D. Epidemiological and clinical features of primary herpes simplex virus ocular infection. Br J Ophthalmol. 1985 Jan;69(1):2–6. doi: 10.1136/bjo.69.1.2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gardner P. S., McQuillin J., Black M. M., Richardson J. Rapid diagnosis of herpesvirus hominis infections in superficial lesions by immunofluorescent antibody technics. Br Med J. 1968 Oct 12;4(5623):89–92. doi: 10.1136/bmj.4.5623.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gordon F. B., Harper I. A., Quan A. L., Treharne J. D., Dwyer R. S., Garland J. A. Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation. J Infect Dis. 1969 Oct;120(4):451–462. doi: 10.1093/infdis/120.4.451. [DOI] [PubMed] [Google Scholar]
- Liu C., Llanes-Rodas R. Application of the immunofluorescent technic to the study of pathogenesis and rapid diagnosis of viral infections. Am J Clin Pathol. 1972 Jun;57(6):829–834. doi: 10.1093/ajcp/57.6.829. [DOI] [PubMed] [Google Scholar]
- Moore D. F. Comparison of human fibroblast cells and primary rabbit kidney cells for isolation of herpes simplex virus. J Clin Microbiol. 1984 Apr;19(4):548–549. doi: 10.1128/jcm.19.4.548-549.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pavan-Langston D. Diagnosis and management of herpes simplex ocular infection. Int Ophthalmol Clin. 1975 Winter;15(4):19–35. doi: 10.1097/00004397-197501540-00004. [DOI] [PubMed] [Google Scholar]
- Schwartz H. S., Vastine D. W., Yamashiroya H., West C. E. Immunofluorescent detection of adenovirus antigen in epidemic keratoconjunctivitis. Invest Ophthalmol. 1976 Mar;15(3):199–207. [PubMed] [Google Scholar]