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. 1986 Sep;70(9):677–680. doi: 10.1136/bjo.70.9.677

Rapid diagnosis of Chlamydia trachomatis infection in patients attending an ophthalmic casualty department.

M J Potts, I D Paul, A P Roome, E O Caul
PMCID: PMC1040798  PMID: 3530317

Abstract

Direct immunofluorescence (DIF) techniques were compared with conventional cell culture for the diagnosis of ocular infections with Chlamydia trachomatis. The DIF test was found to have a sensitivity of 100% and a specificity of 97.5%. Of 178 patients studied, 19 patients (11%) were positive by DIF and 15 (8.4%) by conventional cell culture technique. Four patients who had previous treatment with chloramphenicol eye drops were negative by cell culture but positive by the DIF test. The DIF test is considered to be a rapid, accurate test with a number of advantages over culture techniques for the detection of C. trachomatis. The importance of appropriate referral of positive patients to prevent more serious sequelae due to C. trachomatis infection is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander I., Paul I. D., Caul E. O. Evaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence. Genitourin Med. 1985 Aug;61(4):252–254. doi: 10.1136/sti.61.4.252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beem M. O., Saxon E. M. Respiratory-tract colonization and a distinctive pneumonia syndrome in infants infected with Chlamydia trachomatis. N Engl J Med. 1977 Feb 10;296(6):306–310. doi: 10.1056/NEJM197702102960604. [DOI] [PubMed] [Google Scholar]
  3. Clarke S. K., Hart J. C., Barnard D. L. The disinfection of instruments and hands during outbreaks of epidemic keratoconjunctivitis. Trans Ophthalmol Soc U K. 1972;92:613–618. [PubMed] [Google Scholar]
  4. Conway D., Glazener C. M., Caul E. O., Hodgson J., Hull M. G., Clarke S. K., Stirrat G. M. Chlamydial serology in fertile and infertile women. Lancet. 1984 Jan 28;1(8370):191–193. doi: 10.1016/s0140-6736(84)92113-5. [DOI] [PubMed] [Google Scholar]
  5. Dunlop E. M. Chlamydial genital infection and its complications. Br J Hosp Med. 1983 Jan;29(1):6–11. [PubMed] [Google Scholar]
  6. Dunlop E. M., Harris R. J., Darougar S., Treharne J. D., Al-Egaily S. S. Subclinical pneumonia due to serotypes D-K of Chlamydia trachomatis. Case reports of two infants. Br J Vener Dis. 1980 Oct;56(5):337–340. doi: 10.1136/sti.56.5.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Markham R. H., Richmond S. J., Walshaw N. W., Easty D. L. Severe persistent inclusion conjunctivitis in a young child. Am J Ophthalmol. 1977 Mar;83(3):414–416. doi: 10.1016/0002-9394(77)90742-5. [DOI] [PubMed] [Google Scholar]
  8. Märdh P. A., Lind I., Svensson L., Weström L., Møller B. R. Antibodies to Chlamydia trachomatis, Mycoplasma hominis, and Neisseria gonorrhoeae in sera from patients with acute salpingitis. Br J Vener Dis. 1981 Apr;57(2):125–129. doi: 10.1136/sti.57.2.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mårdh P. A., Ripa T., Svensson L., Weström L. Chilamydia trachomatis infection in patients with acute salpingitis. N Engl J Med. 1977 Jun 16;296(24):1377–1379. doi: 10.1056/NEJM197706162962403. [DOI] [PubMed] [Google Scholar]
  10. Rees E., Tait I. A., Hobson D., Karayiannis P., Lee N. Persistence of chlamydial infection after treatment for neonatal conjunctivitis. Arch Dis Child. 1981 Mar;56(3):193–198. doi: 10.1136/adc.56.3.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Richmond S. J., Paul I. D., Taylor P. K. Value and feasibility of screening women attending STD clinics for cervical chlamydial infections. Br J Vener Dis. 1980 Apr;56(2):92–95. doi: 10.1136/sti.56.2.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Richmond S., Burman R., Crosdale E., Cropper L., Longson D., Enoch B. E., Dodd C. L. A large outbreak of keratoconjunctivitis due to adenovirus type 8. J Hyg (Lond) 1984 Oct;93(2):285–291. doi: 10.1017/s0022172400064810. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ridgway G. L., Oriel J. D. Bacterial infection in the newborn. Br Med J. 1980 Apr 19;280(6221):1087–1087. doi: 10.1136/bmj.280.6221.1087-a. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schachter J. Chlamydial infections (third of three parts). N Engl J Med. 1978 Mar 9;298(10):540–549. doi: 10.1056/NEJM197803092981005. [DOI] [PubMed] [Google Scholar]
  15. Tam M. R., Stamm W. E., Handsfield H. H., Stephens R., Kuo C. C., Holmes K. K., Ditzenberger K., Krieger M., Nowinski R. C. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. doi: 10.1056/NEJM198405033101803. [DOI] [PubMed] [Google Scholar]
  16. Thomas B. J., Evans R. T., Hawkins D. A., Taylor-Robinson D. Sensitivity of detecting Chlamydia trachomatis elementary bodies in smears by use of a fluorescein labelled monoclonal antibody: comparison with conventional chlamydial isolation. J Clin Pathol. 1984 Jul;37(7):812–816. doi: 10.1136/jcp.37.7.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tullo A. B., Richmond S. J., Easty D. L. The presentation and incidence of paratrachoma in adults. J Hyg (Lond) 1981 Aug;87(1):63–69. doi: 10.1017/s0022172400069242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wishart P. K., James C., Wishart M. S., Darougar S. Prevalence of acute conjunctivitis caused by chlamydia, adenovirus, and herpes simplex virus in an ophthalmic casualty department. Br J Ophthalmol. 1984 Sep;68(9):653–655. doi: 10.1136/bjo.68.9.653. [DOI] [PMC free article] [PubMed] [Google Scholar]

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