Abstract
The clinical and laboratory results with a modified Minnesota system of organ culture corneal preservation are presented. A refinement of our preservation technique using a closed system, as well as the addition of chondroitin sulphate to the medium is presented. Laboratory results show preservation of corneal endothelial integrity for at least 21 days with maintenance of normal corneal thickness. In addition, a 10-day quarantine system reduces the risk of donor contamination and secondary endophthalmitis. Preliminary results of the 34 degrees C and 4 degrees C closed Minnesota corneal preservation system using chondroitin sulphate show that it is safe and efficacious and allows intermediate to long-term maintenance of sterile thin tissue prior to corneal transplantation.
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- Aquavella J. V., Van Horn D. L., Haggerty C. J. Corneal preservation using M-K medium. Am J Ophthalmol. 1975 Nov;80(5):791–799. doi: 10.1016/0002-9394(75)90273-1. [DOI] [PubMed] [Google Scholar]
- Bigar F., Kaufman H. E., McCarey B. E., Binder P. S. Improved corneal storage for penetrating keratoplasties in man. Am J Ophthalmol. 1975 Jan;79(1):115–120. doi: 10.1016/0002-9394(75)90467-5. [DOI] [PubMed] [Google Scholar]
- Bourne W. M., Doughman D. J., Lindstrom R. L. Organ-cultured corneal endothelium in vivo. Arch Ophthalmol. 1977 Oct;95(10):1818–1819. doi: 10.1001/archopht.1977.04450100120016. [DOI] [PubMed] [Google Scholar]
- Doughman D. J., Harris J. E., Schmitt M. K. Penetrating keratoplasty using 37 C organ cultured cornea. Trans Sect Ophthalmol Am Acad Ophthalmol Otolaryngol. 1976 Sep-Oct;81(5):778–793. [PubMed] [Google Scholar]
- Doughman D. J., Miller G. E., Mindrup E. A., Harris J. E. The fate of experimental organ-cultured corneal xenografts. Transplantation. 1976 Aug;22(2):132–137. doi: 10.1097/00007890-197608000-00008. [DOI] [PubMed] [Google Scholar]
- Doughman D. J. Prolonged donor cornea preservation in organ culture: long-term clinical evaluation. Trans Am Ophthalmol Soc. 1980;78:567–628. [PMC free article] [PubMed] [Google Scholar]
- Doughman D. J., Van Horn D., Harris J. E., Miller G. E., Lindstrom R., Good R. A. The ultrastructure of human organ-cultured cornea. I. Endothelium. Arch Ophthalmol. 1974 Dec;92(6):516–523. doi: 10.1001/archopht.1974.01010010530015. [DOI] [PubMed] [Google Scholar]
- Doughman D. J., Van Horn D., Harris J. E., Miller G. E., Lindstrom R., Summerlin W., Good R. A. Endothelium of the human organ cultured cornea: an electron microscopic study. Trans Am Ophthalmol Soc. 1973;71:304–328. [PMC free article] [PubMed] [Google Scholar]
- Doughman D. J., Van Horn D., Rodman W. P., Byrnes P., Lindstrom R. L. Human corneal endothelial layer repair during organ culture. Arch Ophthalmol. 1976 Oct;94(10):1791–1796. doi: 10.1001/archopht.1976.03910040565016. [DOI] [PubMed] [Google Scholar]
- Kaufman H. E. Corneal cryopreservation and its clinical application. Transplant Proc. 1976 Jun;8(2 Suppl 1):149–152. [PubMed] [Google Scholar]
- Larsen P. A., Lindstrom R. L., Doughman D. J. Torulopsis glabrata endophthalmitis after keratoplasty with an organ-cultured cornea. Arch Ophthalmol. 1978 Jun;96(6):1019–1022. doi: 10.1001/archopht.1978.03910050543010. [DOI] [PubMed] [Google Scholar]
- Leveille A. S., McMullan F. D., Cavanagh H. D. Endophthalmitis following penetrating keratoplasty. Ophthalmology. 1983 Jan;90(1):38–39. doi: 10.1016/s0161-6420(83)34601-7. [DOI] [PubMed] [Google Scholar]
- Lindstrom R. L., Doughman D. J., Mindrup E. Penetrating keratoplasty following intermediate term organ culture corneal preservation at 37 degrees C. Fortschr Ophthalmol. 1983;80(6):468–471. [PubMed] [Google Scholar]
- Nelson J. D., Mindrup E. A., Chung C. K., Lindstrom R. L., Doughman D. J. Fungal contamination in organ culture. Arch Ophthalmol. 1983 Feb;101(2):280–283. doi: 10.1001/archopht.1983.01040010282020. [DOI] [PubMed] [Google Scholar]
- STOCKER F. W. The endothelium of the cornea and its clinical implications. Trans Am Ophthalmol Soc. 1953;51:669–786. [PMC free article] [PubMed] [Google Scholar]
- Slappey T. E. Corneal preservation. Transplant Proc. 1976 Jun;8(2 Suppl 1):223–227. [PubMed] [Google Scholar]
- Stark W. J., Maumenee A. E., Kenyon K. R. Intermediate-term corneal storage for penetrating keratoplasty. Am J Ophthalmol. 1975 May;79(5):795–802. doi: 10.1016/0002-9394(75)90739-4. [DOI] [PubMed] [Google Scholar]
- Van Horn D. L., Doughman D. J., Harris J. E., Miller G. E., Lindstrom R., Good R. A. Ultrastructure of human organ-cultured cornea. II. Stroma and epithelium. Arch Ophthalmol. 1975 Apr;93(4):275–277. doi: 10.1001/archopht.1975.01010020285007. [DOI] [PubMed] [Google Scholar]