Abstract
A new corneal preserving medium (K-Sol), developed by Kaufman and others, contains purified chondroitin sulphate, TC 199, HEPES buffer, and gentamicin. Another new medium (JM) containing bicarbonate-free glucose-phosphate Ringer solution and dextran 70 has been developed in Japan. New Zealand white rabbit corneas with scleral rims were stored in each medium at 4 degrees C for one or two weeks. The condition of the endothelium was evaluated histologically. Corneas preserved in both media were in good condition at the end of one week. Corneas preserved in K-Sol for two weeks showed fewer endothelial changes than similar tissue stored in JM for two weeks. Corneal swelling was also less in corneas preserved in K-Sol, than in corneas preserved in JM.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bigar F., McCarey B. E., Kaufman H. E. Improved corneal storage: penetrating keratoplasties in rabbits. Exp Eye Res. 1975 Mar;20(3):219–222. doi: 10.1016/0014-4835(75)90135-9. [DOI] [PubMed] [Google Scholar]
- Kaufman H. E., Varnell E. D., Kaufman S., Beuerman R. W., Barron B. A. K-Sol corneal preservation. Am J Ophthalmol. 1985 Aug 15;100(2):299–304. doi: 10.1016/0002-9394(85)90796-2. [DOI] [PubMed] [Google Scholar]
- Kaufman H. E., Varnell E. D., Kaufman S. Chondroitin sulfate in a new cornea preservation medium. Am J Ophthalmol. 1984 Jul 15;98(1):112–114. doi: 10.1016/0002-9394(84)90198-3. [DOI] [PubMed] [Google Scholar]
- Mayes K. R., Graham M. V., Hodson S. The deleterious effects of exogenous bicarbonate on the rabbit cornea undergoing prolonged refrigerated storage. Exp Eye Res. 1978 May;26(5):555–560. doi: 10.1016/0014-4835(78)90065-9. [DOI] [PubMed] [Google Scholar]
- McCarey B. E., Kaufman H. E. Improved corneal storage. Invest Ophthalmol. 1974 Mar;13(3):165–173. [PubMed] [Google Scholar]
- Mizukawa T., Manabe R. [Recent advances in keratoplasty, with special reference to the advantages of liquid preservation]. Nihon Ganka Kiyo. 1968 Dec;19(12):1310–1318. [PubMed] [Google Scholar]
- Sakimoto T., Valenti J., Itoi M., Kaufman H. E. Intermediate-term corneal storage. Invest Ophthalmol. 1974 Mar;13(3):219–228. [PubMed] [Google Scholar]
- Tanishima T., Matsumura Y., Masuda K. [The effect of new intraocular irrigating solution on the rabbit corneal endothelium]. Nippon Ganka Gakkai Zasshi. 1984 May;88(5):829–837. [PubMed] [Google Scholar]
- Van Horn D. L., Schultz R. O. Corneal preservation: recent advances. Surv Ophthalmol. 1977 Jan-Feb;21(4):301–312. doi: 10.1016/0039-6257(77)90112-6. [DOI] [PubMed] [Google Scholar]