Figure 1.

Study design

An intervention study in 6–8 weeks old female BALB/c mice with nine vaccination groups and 6 mice per group was designed. Each mouse was injected intramuscularly with the vaccine formulation three times in a two-week interval at day 0, day 14, and day 28 prior to which blood was collected from the tail vein on the same days. Mice euthanization and final blood collection through cardiac puncture was done at two weeks after the third dose at day 42.