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. 1988 Nov;72(11):874–879. doi: 10.1136/bjo.72.11.874

Detection and quantification of S-100 protein in ocular tissues and fluids from patients with intraocular melanoma.

A J Cochran 1, G N Holland 1, R E Saxton 1, B E Damato 1, W R Foulds 1, H R Herschman 1, R Y Foos 1, B R Straatsma 1, W R Lee 1
PMCID: PMC1041610  PMID: 3207666

Abstract

S-100 protein is a 21,000 dalton acidic calcium-binding protein present in ocular melanomas and some normal ocular tissues. Ocular fluids and extracts of ocular tumours were examined by a sensitive radioimmunoassay that could detect less than 5 ng of S-100 protein in minute volumes of fluid. Three ocular melanoma biopsy specimens had S-100 protein at levels between 25 and 1300 ng/ml, comparable to that found in a cutaneous melanoma biopsy specimen (1000 ng/ml). (SI conversion: ng/ml = microgram/l.) Six melanoma culture lines had 1000 to 125,000 ng/ml. Four lymphoblastoid cultures had less than 2 ng/ml, and three colon carcinoma cultures had 180 ng/ml. Subretinal fluid from 23 melanoma-containing eyes had 10 to 76,800 ng/ml. Lesser amounts were found in eyes with small, anteriorly located, lightly pigmented tumours. Vitreous from 3 melanoma-containing eyes had 10,000 to 11,000 ng/ml. Vitreous obtained from three eyes during tractional retinal detachment repair had 500 to 1600 ng/ml, and vitreous obtained at necropsy from six normal eyes had 2 to 120 ng/ml. Aqueous from six melanoma-containing eyes had 10 to 30 ng/ml, levels not significantly different from those observed in three normal eyes (80-120 ng/ml). This approach provides new insight into the interaction of ocular tumours and adjacent ocular fluids and may, with more specific tumour markers, have diagnostic applications.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bock E. Nervous system specific proteins. J Neurochem. 1978 Jan;30(1):7–14. doi: 10.1111/j.1471-4159.1978.tb07028.x. [DOI] [PubMed] [Google Scholar]
  2. Burk M. W. Monoclonal hybridoma antibodies in human melanoma: current status. Int Adv Surg Oncol. 1984;7:241–270. [PubMed] [Google Scholar]
  3. Cocchia D., Michetti F. S-100 antigen in satellite cells of the adrenal medulla and the superior cervical ganglion of the rat. An immunochemical and immunocytochemical study. Cell Tissue Res. 1981;215(1):103–112. doi: 10.1007/BF00236252. [DOI] [PubMed] [Google Scholar]
  4. Cochran A. J., Foulds W. S., Damato B. E., Trope G. E., Morrison L., Lee W. R. Assessment of immunological techniques in the diagnosis and prognosis of ocular malignant melanoma. Br J Ophthalmol. 1985 Mar;69(3):171–176. doi: 10.1136/bjo.69.3.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cochran A. J., Holland G. N., Wen D. R., Herschman H. R., Lee W. R., Foos R. Y., Straatsma B. R. Detection of cytoplasmic S-100 protein in primary and metastatic intraocular melanomas. Invest Ophthalmol Vis Sci. 1983 Aug;24(8):1153–1155. [PubMed] [Google Scholar]
  6. Cochran A. J., Wen D. R. S-100 protein as a marker for melanocytic and other tumours. Pathology. 1985 Apr;17(2):340–345. doi: 10.3109/00313028509063777. [DOI] [PubMed] [Google Scholar]
  7. Davidorf F. H., Letson A. D., Weiss E. T., Levine E. Incidence of misdiagnosed and unsuspected choroidal melanomas. A 50-year experience. Arch Ophthalmol. 1983 Mar;101(3):410–412. doi: 10.1001/archopht.1983.01040010410013. [DOI] [PubMed] [Google Scholar]
  8. Fan K. S-100 protein synthesis in cultured glioma cell is G1-phase of cell cycle dependent. Brain Res. 1982 Apr 15;237(2):498–503. doi: 10.1016/0006-8993(82)90462-0. [DOI] [PubMed] [Google Scholar]
  9. Gaynor R., Irie R., Morton D., Herschman H. R. S100 protein is present in cultured human malignant melanomas. Nature. 1980 Jul 24;286(5771):400–401. doi: 10.1038/286400a0. [DOI] [PubMed] [Google Scholar]
  10. Isobe T., Ishioka N., Okuyama T. Structural relation of two S-100 proteins in bovine brain; subunit composition of S-100a protein. Eur J Biochem. 1981 Apr;115(3):469–474. doi: 10.1111/j.1432-1033.1981.tb06225.x. [DOI] [PubMed] [Google Scholar]
  11. Kanamori M., Endo T., Shirakawa S., Sakurai M., Hidaka H. S-100 antigen in human T lymphocytes. Biochem Biophys Res Commun. 1982 Oct 29;108(4):1447–1453. doi: 10.1016/s0006-291x(82)80069-7. [DOI] [PubMed] [Google Scholar]
  12. Kondo H., Iwanaga T., Nakajima T. Immunocytochemical study on the localization of neuron-specific enolase and S-100 protein in the carotid body of rats. Cell Tissue Res. 1982;227(2):291–295. doi: 10.1007/BF00210887. [DOI] [PubMed] [Google Scholar]
  13. Michetti F., Dell'Anna E., Tiberio G., Cocchia D. Immunochemical and immunocytochemical study of S-100 protein in rat adipocytes. Brain Res. 1983 Mar 7;262(2):352–356. doi: 10.1016/0006-8993(83)91032-6. [DOI] [PubMed] [Google Scholar]
  14. Moore B. W. A soluble protein characteristic of the nervous system. Biochem Biophys Res Commun. 1965 Jun 9;19(6):739–744. doi: 10.1016/0006-291x(65)90320-7. [DOI] [PubMed] [Google Scholar]
  15. Moore B. W. Chemistry and biology of two proteins, S-100 and 14-3-2, specific to the nervous system. Int Rev Neurobiol. 1972;15:215–225. doi: 10.1016/s0074-7742(08)60332-3. [DOI] [PubMed] [Google Scholar]
  16. Møller M., Ingild A., Bock E. Immunohistochemical demonstration of S-100 protein and GFA protein in interstitial cells of rat pineal gland. Brain Res. 1978 Jan 20;140(1):1–13. doi: 10.1016/0006-8993(78)90234-2. [DOI] [PubMed] [Google Scholar]
  17. Nakajima T., Watanabe S., Sato Y., Kameya T., Shimosato Y. Immunohistochemical demonstration of S100 protein in human malignant melanoma and pigmented nevi. Gan. 1981 Apr;72(2):335–336. [PubMed] [Google Scholar]
  18. Nakajima T., Watanabe S., Sato Y., Shimosato Y., Motoi M., Lennert K. S-100 protein in Langerhans cells, interdigitating reticulum cells and histiocytosis X cells. Gan. 1982 Jun;73(3):429–432. [PubMed] [Google Scholar]
  19. Nakajima T., Yamaguchi H., Takahashi K. S100 protein in folliculostellate cells of the rat pituitary anterior lobe. Brain Res. 1980 Jun 9;191(2):523–531. doi: 10.1016/0006-8993(80)91300-1. [DOI] [PubMed] [Google Scholar]
  20. Stefansson K., Wollmann R. L., Moore B. W., Arnason B. G. S-100 protein in human chondrocytes. Nature. 1982 Jan 7;295(5844):63–64. doi: 10.1038/295063a0. [DOI] [PubMed] [Google Scholar]
  21. Stefansson K., Wollmann R. L., Moore B. W. Distribution of S-100 protein outside the central nervous system. Brain Res. 1982 Feb 25;234(2):309–317. doi: 10.1016/0006-8993(82)90871-x. [DOI] [PubMed] [Google Scholar]
  22. Terenghi G., Cocchia D., Michetti F., Diani A. R., Peterson T., Cole D. F., Bloom S. R., Polak J. M. Localization of S-100 protein in Müller cells of the retina--1. Light microscopical immunocytochemistry. Invest Ophthalmol Vis Sci. 1983 Jul;24(7):976–980. [PubMed] [Google Scholar]
  23. Zuckerman J. E., Herschman H. R., Levine L. Appearance of a brain specific antigen (th S-100 protein) during human foetal development. J Neurochem. 1970 Feb;17(2):247–251. doi: 10.1111/j.1471-4159.1970.tb02207.x. [DOI] [PubMed] [Google Scholar]

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