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. 1989 Apr;73(4):276–282. doi: 10.1136/bjo.73.4.276

Immunopathology of trachomatous conjunctivitis.

A M el-Asrar 1, J J Van den Oord 1, K Geboes 1, L Missotten 1, M H Emarah 1, V Desmet 1
PMCID: PMC1041713  PMID: 2713305

Abstract

Upper palpebral conjunctival biopsy specimens obtained from eight patients with active trachoma were examined by routine histological and immunohistochemical methods. The epithelium expressed class I major histocompatibility complex (MHC) products throughout and class II MHC products in the superficial layers. The epithelial inflammatory infiltrate consisted of polymorphonuclear leucocytes, macrophages, T lymphocytes, and dendritic cells. In the underlying stroma the inflammatory infiltrate was organised as B lymphoid follicles, and there was also a diffuse infiltrate consisting of plasma cells and scattered B lymphoid cells, dendritic cells, T cells, macrophages, and polymorphonuclear leucocytes. Each type of cell has its special location in the tissue. Plasma cells were located on a subepithelial band and as a dense infiltrate round the acini of accessory lacrimal glands. IgA+ plasma cells outnumbered IgG+ cells, whereas IgM+ and IgE+ cells were few. Our data provide good evidence for the presence of both humoral and cell mediated immune responses and a possible role for autoimmune mechanisms in the conjunctival tissues of trachoma patients.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams T. E., Bodmer J. G., Bodmer W. F. Production and characterization of monoclonal antibodies recognizing the alpha-chain subunits of human ia alloantigens. Immunology. 1983 Dec;50(4):613–624. [PMC free article] [PubMed] [Google Scholar]
  2. Andersen P., Møller B. R. Autoantibodies in patients with acute salpingitis caused by Chlamydia trachomatis. Scand J Infect Dis. 1982;14(1):19–21. doi: 10.3109/inf.1982.14.issue-1.04. [DOI] [PubMed] [Google Scholar]
  3. Bard J., Levitt D. Chlamydia trachomatis stimulates human peripheral blood B lymphocytes to proliferate and secrete polyclonal immunoglobulins in vitro. Infect Immun. 1984 Jan;43(1):84–92. doi: 10.1128/iai.43.1.84-92.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Basham T. Y., Nickoloff B. J., Merigan T. C., Morhenn V. B. Recombinant gamma interferon differentially regulates class II antigen expression and biosynthesis on cultured normal human keratinocytes. J Interferon Res. 1985 Winter;5(1):23–32. doi: 10.1089/jir.1985.5.23. [DOI] [PubMed] [Google Scholar]
  5. Bottazzo G. F., Dean B. M., McNally J. M., MacKay E. H., Swift P. G., Gamble D. R. In situ characterization of autoimmune phenomena and expression of HLA molecules in the pancreas in diabetic insulitis. N Engl J Med. 1985 Aug 8;313(6):353–360. doi: 10.1056/NEJM198508083130604. [DOI] [PubMed] [Google Scholar]
  6. Bottazzo G. F., Pujol-Borrell R., Hanafusa T., Feldmann M. Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity. Lancet. 1983 Nov 12;2(8359):1115–1119. doi: 10.1016/s0140-6736(83)90629-3. [DOI] [PubMed] [Google Scholar]
  7. Darougar S., Jones B. R. Trachoma. Br Med Bull. 1983 Apr;39(2):117–122. doi: 10.1093/oxfordjournals.bmb.a071801. [DOI] [PubMed] [Google Scholar]
  8. Darougar S. The humoral immune response to chlamydial infection in humans. Rev Infect Dis. 1985 Nov-Dec;7(6):726–730. doi: 10.1093/clinids/7.6.726. [DOI] [PubMed] [Google Scholar]
  9. Darougar S., Treharne J. D., Minassian D., El-Sheikh H., Dines R. J., Jones B. R. Rapid serological test for diagnosis of chlamydial ocular infections. Br J Ophthalmol. 1978 Aug;62(8):503–508. doi: 10.1136/bjo.62.8.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dawson C. R. How does the external eye resist infection. Invest Ophthalmol. 1976 Dec;15(12):971–974. [PubMed] [Google Scholar]
  11. Duc-Goiran P., Zernicki L. Interferons induced by Chlamydia trachomatis in human lymphocyte cultures. Biomedicine. 1981 Sep;34(2):88–93. [PubMed] [Google Scholar]
  12. Epstein A. L., Marder R. J., Winter J. N., Fox R. I. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984 Aug;133(2):1028–1036. [PubMed] [Google Scholar]
  13. Franklin R. M., Remus L. E. Conjunctival-associated lymphoid tissue: evidence for a role in the secretory immune system. Invest Ophthalmol Vis Sci. 1984 Feb;25(2):181–187. [PubMed] [Google Scholar]
  14. Geboes K., Abu el-Asrar A., Missotten L. Immune cells in a case of postherpetic marginal trophic ulcer. Br J Ophthalmol. 1989 Mar;73(3):191–196. doi: 10.1136/bjo.73.3.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gillette T. E., Chandler J. W., Greiner J. V. Langerhans cells of the ocular surface. Ophthalmology. 1982 Jun;89(6):700–711. doi: 10.1016/s0161-6420(82)34737-5. [DOI] [PubMed] [Google Scholar]
  16. Hanafusa T., Pujol-Borrell R., Chiovato L., Russell R. C., Doniach D., Bottazzo G. F. Aberrant expression of HLA-DR antigen on thyrocytes in Graves' disease: relevance for autoimmunity. Lancet. 1983 Nov 12;2(8359):1111–1115. doi: 10.1016/s0140-6736(83)90628-1. [DOI] [PubMed] [Google Scholar]
  17. Johnson A. P. Pathogenesis and immunology of chlamydial infections of the genital tract. Rev Infect Dis. 1985 Nov-Dec;7(6):741–745. doi: 10.1093/clinids/7.6.741. [DOI] [PubMed] [Google Scholar]
  18. Levitt D., Danen R. Development and regulation of chlamydia-responsive murine B lymphocytes. J Immunol. 1987 May 15;138(10):3468–3474. [PubMed] [Google Scholar]
  19. Londei M., Lamb J. R., Bottazzo G. F., Feldmann M. Epithelial cells expressing aberrant MHC class II determinants can present antigen to cloned human T cells. Nature. 1984 Dec 13;312(5995):639–641. doi: 10.1038/312639a0. [DOI] [PubMed] [Google Scholar]
  20. Murray E. S., Charbonnet L. T., MacDonald A. B. Immunity to chlamydial infections of the eye. I. The role of circulatory and secretory antibodies in resistance to reinfection with guinea pig inclusion conjunctivitis. J Immunol. 1973 Jun;110(6):1518–1525. [PubMed] [Google Scholar]
  21. Patton D. L., Taylor H. R. The histopathology of experimental trachoma: ultrastructural changes in the conjunctival epithelium. J Infect Dis. 1986 May;153(5):870–878. doi: 10.1093/infdis/153.5.870. [DOI] [PubMed] [Google Scholar]
  22. Poppema S., Hollema H., Visser L., Vos H. Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections. Am J Pathol. 1987 Jun;127(3):418–429. [PMC free article] [PubMed] [Google Scholar]
  23. Sacks D. L., Todd W. J., Macdonald A. B. Cell-mediated immune responses in owl monkeys (Aotus trivirgatus) with trachoma to soluble antigens of Chlamydia trachomatis. Clin Exp Immunol. 1978 Jul;33(1):57–64. [PMC free article] [PubMed] [Google Scholar]
  24. Silberberg-Sinakin I., Thorbecke G. J., Baer R. L., Rosenthal S. A., Berezowsky V. Antigen-bearing langerhans cells in skin, dermal lymphatics and in lymph nodes. Cell Immunol. 1976 Aug;25(2):137–151. doi: 10.1016/0008-8749(76)90105-2. [DOI] [PubMed] [Google Scholar]
  25. Tabibzadeh S. S., Gerber M. A., Satyaswaroop P. G. Induction of HLA-DR antigen expression in human endometrial epithelial cells in vitro by recombinant gamma-interferon. Am J Pathol. 1986 Oct;125(1):90–96. [PMC free article] [PubMed] [Google Scholar]
  26. Taylor H. R., Johnson S. L., Prendergast R. A., Schachter J., Dawson C. R., Silverstein A. M. An animal model of trachoma II. The importance of repeated reinfection. Invest Ophthalmol Vis Sci. 1982 Oct;23(4):507–515. [PubMed] [Google Scholar]
  27. Taylor H. R. Ocular models of chlamydial infection. Rev Infect Dis. 1985 Nov-Dec;7(6):737–740. doi: 10.1093/clinids/7.6.737. [DOI] [PubMed] [Google Scholar]
  28. Thorsby E., Berle E., Nousiainen H. HLA-D region molecules restrict proliferative T cell responses to antigen. Immunol Rev. 1982;66:39–56. doi: 10.1111/j.1600-065x.1982.tb00433.x. [DOI] [PubMed] [Google Scholar]
  29. Treharne J. D., Dwyer R. S., Darougar S., Jones B. R., Daghfous T. Antichlamydial antibody in tears and sera, and serotypes of Chlamydia trachomatis isolated from schoolchildren in Southern Tunisia. Br J Ophthalmol. 1978 Aug;62(8):509–515. doi: 10.1136/bjo.62.8.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Part Two: symbiotic relationship between lymphocytes and macrophages. Adv Immunol. 1981;31:1–136. doi: 10.1016/s0065-2776(08)60919-0. [DOI] [PubMed] [Google Scholar]
  31. Van den Oord J. J., Sciot R., Desmet V. J. Expression of MHC products by normal and abnormal bile duct epithelium. J Hepatol. 1986;3(3):310–317. doi: 10.1016/s0168-8278(86)80483-4. [DOI] [PubMed] [Google Scholar]
  32. Watson R. R., MacDonald A. B., Murray E. S., Modabber F. Z. Immunity to chlamydial infections of the eye. 3. Presence and duration of delayed hypersensitivity to guinea pig inclusion conjuctivitis. J Immunol. 1973 Aug;111(2):618–623. [PubMed] [Google Scholar]
  33. Williams D. M., Schachter J. Role of cell-mediated immunity in chlamydial infection: implications for ocular immunity. Rev Infect Dis. 1985 Nov-Dec;7(6):754–759. doi: 10.1093/clinids/7.6.754. [DOI] [PubMed] [Google Scholar]
  34. Young E., Taylor H. R. Immune mechanisms in chlamydial eye infection: cellular immune responses in chronic and acute disease. J Infect Dis. 1984 Nov;150(5):745–751. doi: 10.1093/infdis/150.5.745. [DOI] [PubMed] [Google Scholar]

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