Figure 7: SidC/SdcA is required for small GTPase ubiquitination beyond the Rab subfamily.

(A) Immunoblot analysis of small GTPase ubiquitination during infection with ΔsidC/sdcA L.p. strain panel (WT, ΔdotA, ΔsidC/sdcA, and ΔsidC/sdcA transformed with vector or plasmid expressing SdcA or SidC). HEK293T FcγR cells transfected with the indicated GFP-tagged small GTPase were infected (MOI=50) with the indicated strain or left uninfected. Cells were lysed at 1 hour post infection and probed with anti-GFP and anti-Hsp70 antibodies. (B) Comparative analysis of literature reports of small GTPase recruitment to the LCV with small GTPases found to be ubiquitinated during infection in our proteomic analysis. (C) Schematic of models for downstream consequences and mechanism of SidC/SdcA mediated small GTPase ubiquitination.