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. 1990 May;74(5):261–264. doi: 10.1136/bjo.74.5.261

Manipulative damage to the endothelium of infant and adult donor corneas.

I N Nartey 1, E S Sherrard 1, A D Steele 1
PMCID: PMC1042092  PMID: 2354131

Abstract

Six human donor corneas were studied with the scanning electron microscope to quantify the hazards to the endothelium during the excision of corneoscleral buttons. Although the number studied was small, it was found that: (1) striae were more numerous in the flaccid, very young, donor corneas (under 1 year old) than in the more rigid adult corneas: (2) iridocorneal endothelial touch can result in loss of the posterior membrane and death of the touched endothelial cells. This corroborates the findings of other investigators. Both findings are important, because these conditions reduce the quality of the donor cornea for transplantation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alfonso E., Tucker G. S., Batlle J. F., Mandelbaum S., Gelender H., Forster R. K. Snailtracks of the corneal endothelium. Ophthalmology. 1986 Mar;93(3):344–349. doi: 10.1016/s0161-6420(86)33736-9. [DOI] [PubMed] [Google Scholar]
  2. Bahn C. F., Grosserode R., Musch D. C., Feder J., Meyer R. F., MacCallum D. K., Lillie J. H., Rich N. M. Effect of 1% sodium hyaluronate (Healon) on a nonregenerating (feline) corneal endothelium. Invest Ophthalmol Vis Sci. 1986 Oct;27(10):1485–1494. [PubMed] [Google Scholar]
  3. Fong L. P., Gladstone D., Casey T. A. Corneo-scleral rim cultures: donor contamination a case of fungal endophthalmitis transmitted by K-Sol stored cornea. Eye (Lond) 1988;2(Pt 6):670–676. doi: 10.1038/eye.1988.123. [DOI] [PubMed] [Google Scholar]
  4. Grutzmacher R. D., Oiland D., McKillop B. R., Bunt-Milam A. H. Donor corneal endothelial striae. Am J Ophthalmol. 1986 Oct 15;102(4):508–515. doi: 10.1016/0002-9394(86)90082-6. [DOI] [PubMed] [Google Scholar]
  5. Kaufman H. E., Capella J. A., Robbins J. E. The human corneal endothelium. Am J Ophthalmol. 1966 May;61(5 Pt 1):835–841. doi: 10.1016/0002-9394(66)90921-4. [DOI] [PubMed] [Google Scholar]
  6. Mac Rae S. M., Edelhauser H. F., Hyndiuk R. A., Burd E. M., Schultz R. O. The effects of sodium hyaluronate, chondroitin sulfate, and methylcellulose on the corneal endothelium and intraocular pressure. Am J Ophthalmol. 1983 Mar;95(3):332–341. doi: 10.1016/s0002-9394(14)78301-1. [DOI] [PubMed] [Google Scholar]
  7. McKinnon J. R., Walters G. D. Cadaver storage time. An important factor in donor cornea survival. Arch Ophthalmol. 1976 Feb;94(2):217–220. doi: 10.1001/archopht.1976.03910030097003. [DOI] [PubMed] [Google Scholar]
  8. Miller D., Weiss J. N. The effect of corneal stretch on the endothelium. Ophthalmic Surg. 1981 Oct;12(10):731–736. [PubMed] [Google Scholar]
  9. Nartey I. N., Ng W., Sherrard E. S., Steele A. D. Posterior corneal folds and endothelial cell damage in human donor eyes. Br J Ophthalmol. 1989 Feb;73(2):121–125. doi: 10.1136/bjo.73.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Norn M. S. Vital staining of corneal endothelium in cataract extraction. Acta Ophthalmol (Copenh) 1971;49(5):725–733. doi: 10.1111/j.1755-3768.1971.tb08670.x. [DOI] [PubMed] [Google Scholar]
  11. Pfister R. R., Breaud S. Aphakic refractive penetrating keratoplasty using newborn donor corneas. A preliminary report on an alternative approach to refractive correction. Ophthalmology. 1983 Oct;90(10):1207–1212. doi: 10.1016/s0161-6420(83)34417-1. [DOI] [PubMed] [Google Scholar]
  12. Roozitalab M. H., Requard J. J., Fogle J. A., Green W. R., Graham C. R., Jr Endothelial stretch striae in donor cornea: cause, significance and prevention. Md State Med J. 1979 May;28(5):47–49. [PubMed] [Google Scholar]
  13. SCHAEFFER E. M. Ultrastructural changes in moist chamber corneas. Invest Ophthalmol. 1963 Jun;2:272–282. [PubMed] [Google Scholar]
  14. Saggau D. D., Bourne W. M. A comparison of two preservation media (CSM and K-Sol) by scanning electron microscopy of preserved corneal endothelium. Arch Ophthalmol. 1989 Mar;107(3):429–432. doi: 10.1001/archopht.1989.01070010439041. [DOI] [PubMed] [Google Scholar]
  15. Sherrard E. S. Characterization of changes observed in the corneal endothelium with the specular microscope. Invest Ophthalmol Vis Sci. 1978 Apr;17(4):322–326. [PubMed] [Google Scholar]
  16. Sherrard E. S., Novakovic P., Speedwell L. Age-related changes of the corneal endothelium and stroma as seen in vivo by specular microscopy. Eye (Lond) 1987;1(Pt 2):197–203. doi: 10.1038/eye.1987.37. [DOI] [PubMed] [Google Scholar]
  17. Steele A. D., Pierse D. Mastering the donor eye: a new device for obtaining donor corneal discs. Br J Ophthalmol. 1978 Feb;62(2):86–88. doi: 10.1136/bjo.62.2.86. [DOI] [PMC free article] [PubMed] [Google Scholar]

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