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. 1991 Jul;75(7):421–424. doi: 10.1136/bjo.75.7.421

Experimental Acanthamoeba keratitis: II. Immunohistochemical evaluation.

D F Larkin 1, D L Easty 1
PMCID: PMC1042410  PMID: 1854696

Abstract

In a Wistar rat experimental model of Acanthamoeba keratitis immunohistochemical techniques were used to analyse the host cellular response. The inflammatory cell profile was observed to change at intervals. In tissue sections the cellular response consisted of neutrophils on the first day but predominantly macrophages on the following days. Some T lymphocytes but no B lymphocytes were observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auran J. D., Starr M. B., Jakobiec F. A. Acanthamoeba keratitis. A review of the literature. Cornea. 1987;6(1):2–26. [PubMed] [Google Scholar]
  2. Burchiel S. W., Billman J. R., Alber T. R. Rapid and efficient purification of mouse monoclonal antibodies from ascites fluid using high performance liquid chromatography. J Immunol Methods. 1984 Apr 13;69(1):33–42. doi: 10.1016/0022-1759(84)90274-6. [DOI] [PubMed] [Google Scholar]
  3. Dallman M. J., Thomas M. L., Green J. R. MRC OX-19: a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses. Eur J Immunol. 1984 Mar;14(3):260–267. doi: 10.1002/eji.1830140311. [DOI] [PubMed] [Google Scholar]
  4. Dijkstra C. D., Döpp E. A., Joling P., Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 1985 Mar;54(3):589–599. [PMC free article] [PubMed] [Google Scholar]
  5. Foster C. S., Monroe J. G., Campbell R., Kalpaxis J., Wetzig R., Greene M. I. Ocular immune responses. II. Priming of A/J mice in the vitreous induces either enhancement of or suppression of subsequent hapten-specific DTH responses. J Immunol. 1986 Apr 15;136(8):2787–2791. [PubMed] [Google Scholar]
  6. Hsiung L., Barclay A. N., Brandon M. R., Sim E., Porter R. R. Purification of human C3b inactivator by monoclonal-antibody affinity chromatography. Biochem J. 1982 Apr 1;203(1):293–298. doi: 10.1042/bj2030293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Larkin D. F., Easty D. L. Experimental Acanthamoeba keratitis: I. Preliminary findings. Br J Ophthalmol. 1990 Sep;74(9):551–555. doi: 10.1136/bjo.74.9.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Martínez A. J. Is Acanthamoeba encephalitis an opportunistic infection? Neurology. 1980 Jun;30(6):567–574. doi: 10.1212/wnl.30.6.567. [DOI] [PubMed] [Google Scholar]
  9. Mathers W., Stevens G., Jr, Rodrigues M., Chan C. C., Gold J., Visvesvara G. S., Lemp M. A., Zimmerman L. E. Immunopathology and electron microscopy of Acanthamoeba keratitis. Am J Ophthalmol. 1987 May 15;103(5):626–635. doi: 10.1016/s0002-9394(14)74321-1. [DOI] [PubMed] [Google Scholar]
  10. Moore M. B., McCulley J. P., Newton C., Cobo L. M., Foulks G. N., O'Day D. M., Johns K. J., Driebe W. T., Wilson L. A., Epstein R. J. Acanthamoeba keratitis. A growing problem in soft and hard contact lens wearers. Ophthalmology. 1987 Dec;94(12):1654–1661. [PubMed] [Google Scholar]
  11. Paterson D. J., Jefferies W. A., Green J. R., Brandon M. R., Corthesy P., Puklavec M., Williams A. F. Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts. Mol Immunol. 1987 Dec;24(12):1281–1290. doi: 10.1016/0161-5890(87)90122-2. [DOI] [PubMed] [Google Scholar]
  12. Stehr-Green J. K., Bailey T. M., Visvesvara G. S. The epidemiology of Acanthamoeba keratitis in the United States. Am J Ophthalmol. 1989 Apr 15;107(4):331–336. doi: 10.1016/0002-9394(89)90654-5. [DOI] [PubMed] [Google Scholar]
  13. Woollett G. R., Barclay A. N., Puklavec M., Williams A. F. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes. Eur J Immunol. 1985 Feb;15(2):168–173. doi: 10.1002/eji.1830150211. [DOI] [PubMed] [Google Scholar]
  14. Wright P., Warhurst D., Jones B. R. Acanthamoeba keratitis successfully treated medically. Br J Ophthalmol. 1985 Oct;69(10):778–782. doi: 10.1136/bjo.69.10.778. [DOI] [PMC free article] [PubMed] [Google Scholar]

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