Skip to main content
PMC home page
The British Journal of Ophthalmology logoLink to The British Journal of Ophthalmology
. 1991 Oct;75(10):580–583. doi: 10.1136/bjo.75.10.580

Effect of beta radiation on proliferating human Tenon's capsule fibroblasts.

P T Khaw 1, S Ward 1, I Grierson 1, N S Rice 1
PMCID: PMC1042492  PMID: 1954205

Abstract

The effects of different doses of beta radiation from a strontium-90 source on the proliferation of human Tenon's capsule fibroblasts were studied. The cultured fibroblasts were exposed to doses of 100, 250, 500, 750, 1000, 1500, and 3000 rads, and cell numbers were counted at days 3, 7, and 14. Treatment inhibited the proliferation of the fibroblasts. At seven days the cells exposed to 3000 rads showed a decrease relative to the starting cell numbers, and at 14 days the cells exposed to 1500 and 3000 rads showed a decrease in cell numbers. The doses of radiation which inhibited cell proliferation more than 50% (at day 7 and 14) and yet did not cause a decrease in the cell population were 500, 750, and 1000 rads. beta Radiation reduces the proliferation of human Tenon's capsule fibroblasts, and at higher doses this effect may be more pronounced one and two weeks after irradiation.

Full text

PDF
580

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Addicks E. M., Quigley H. A., Green W. R., Robin A. L. Histologic characteristics of filtering blebs in glaucomatous eyes. Arch Ophthalmol. 1983 May;101(5):795–798. doi: 10.1001/archopht.1983.01040010795021. [DOI] [PubMed] [Google Scholar]
  2. Aswad M. I., Baum J. Optimal time for postoperative irradiation of pterygia. Ophthalmology. 1987 Nov;94(11):1450–1451. doi: 10.1016/s0161-6420(87)33267-1. [DOI] [PubMed] [Google Scholar]
  3. Barron A., McDonald J. E., Hughes W. F. Long-term complications of beta-radiation therapy in ophthalmology. Trans Am Ophthalmol Soc. 1970;68:113–128. [PMC free article] [PubMed] [Google Scholar]
  4. Beauchamp G. R., Parks M. M. Filtering surgery in children: barriers to success. Ophthalmology. 1979 Jan;86(1):170–180. doi: 10.1016/s0161-6420(79)35547-6. [DOI] [PubMed] [Google Scholar]
  5. COHEN L. B., GRAHAM T. F., FRY W. E. Beta radiation; as an adjunct to glaucoma surgery in the Negro. Am J Ophthalmol. 1959 Jan;47(1 Pt 1):54–61. [PubMed] [Google Scholar]
  6. Cameron M. E. Beta irradiation as an adjunct to surgery in refractory glaucoma. Trans Aust Coll Ophthalmol. 1970;2:53–60. [PubMed] [Google Scholar]
  7. Cooper J. S. Postoperative irradiation of pterygia: ten more years of experience. Radiology. 1978 Sep;128(3):753–756. doi: 10.1148/128.3.753. [DOI] [PubMed] [Google Scholar]
  8. Desjardins D. C., Parrish R. K., 2nd, Folberg R., Nevarez J., Heuer D. K., Gressel M. G. Wound healing after filtering surgery in owl monkeys. Arch Ophthalmol. 1986 Dec;104(12):1835–1839. doi: 10.1001/archopht.1986.01050240109050. [DOI] [PubMed] [Google Scholar]
  9. GRILLO H. C., POTSAID M. S. Studies in wound healing. IV. Retardation of contraction by localx-irradiation, and observations relating to the origin of fibroblasts in repair. Ann Surg. 1961 Nov;154:741–750. [PMC free article] [PubMed] [Google Scholar]
  10. Greenberg M. E., Ziff E. B. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature. 1984 Oct 4;311(5985):433–438. doi: 10.1038/311433a0. [DOI] [PubMed] [Google Scholar]
  11. Heuer D. K., Gressel M. G., Parrish R. K., 2nd, Anderson D. R., Hodapp E., Palmberg P. F. Trabeculectomy in aphakic eyes. Ophthalmology. 1984 Sep;91(9):1045–1051. doi: 10.1016/s0161-6420(84)34196-3. [DOI] [PubMed] [Google Scholar]
  12. Hitchings R. A., Grierson I. Clinico pathological correlation in eyes with failed fistulizing surgery. Trans Ophthalmol Soc U K. 1983;103(Pt 1):84–88. [PubMed] [Google Scholar]
  13. Jampel H. D., McGuigan L. J., Dunkelberger G. R., L'Hernault N. L., Quigley H. A. Cellular proliferation after experimental glaucoma filtration surgery. Arch Ophthalmol. 1988 Jan;106(1):89–94. doi: 10.1001/archopht.1988.01060130095036. [DOI] [PubMed] [Google Scholar]
  14. Jay J. L., Murray S. B. Early trabeculectomy versus conventional management in primary open angle glaucoma. Br J Ophthalmol. 1988 Dec;72(12):881–889. doi: 10.1136/bjo.72.12.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Knapp A., Heuer D. K., Stern G. A., Driebe W. T., Jr Serious corneal complications of glaucoma filtering surgery with postoperative 5-fluorouracil. Am J Ophthalmol. 1987 Feb 15;103(2):183–187. doi: 10.1016/s0002-9394(14)74224-2. [DOI] [PubMed] [Google Scholar]
  16. Kondo M., Araie M. Concentration change of fluorouracil in the external segment of the eye after subconjunctival injection. Arch Ophthalmol. 1988 Dec;106(12):1718–1721. doi: 10.1001/archopht.1988.01060140890035. [DOI] [PubMed] [Google Scholar]
  17. MCDONALD J. E., WILDER H. C. The effect of beta radiation on corneal healing. Am J Ophthalmol. 1955 Nov;40(5 Pt 2):170–180. doi: 10.1016/0002-9394(55)91852-3. [DOI] [PubMed] [Google Scholar]
  18. MEAD K. W. Beta radiation for recurrent pterygia. Trans Ophthalmol Soc Aust. 1956;16:101–109. [PubMed] [Google Scholar]
  19. Merritt J. C. Filtering procedures in American blacks. Ophthalmic Surg. 1980 Feb;11(2):91–94. [PubMed] [Google Scholar]
  20. Miller M. H., Grierson I., Unger W. G., Hitchings R. A. The effect of topical dexamethasone and preoperative beta irradiation on a model of glaucoma fistulizing surgery in the rabbit. Ophthalmic Surg. 1990 Jan;21(1):44–54. [PubMed] [Google Scholar]
  21. Nevárez J. A., Parrish R. K., 2nd, Heuer D. K., Hajek A. S., Houdek P. V., Mallick K. S. The effect of beta irradiation on monkey Tenon's capsule fibroblasts in tissue culture. Curr Eye Res. 1987 May;6(5):719–723. doi: 10.3109/02713688709034835. [DOI] [PubMed] [Google Scholar]
  22. Ogino N., Masuda H., Abe Y. [Beta-irradiation in the filtering operation]. Nippon Ganka Gakkai Zasshi. 1966 Nov;70(11):1834–1839. [PubMed] [Google Scholar]
  23. Seetner A., Morin J. D. Healing of trabeculectomies in rabbits. Can J Ophthalmol. 1979 Apr;14(2):121–125. [PubMed] [Google Scholar]
  24. Watson P. G., Grierson I. The place of trabeculectomy in the treatment of glaucoma. Ophthalmology. 1981 Mar;88(3):175–196. doi: 10.1016/s0161-6420(81)35051-9. [DOI] [PubMed] [Google Scholar]
  25. Watson P. G., Jakeman C., Ozturk M., Barnett M. F., Barnett F., Khaw K. T. The complications of trabeculectomy (a 20-year follow-up). Eye (Lond) 1990;4(Pt 3):425–438. doi: 10.1038/eye.1990.54. [DOI] [PubMed] [Google Scholar]

Articles from The British Journal of Ophthalmology are provided here courtesy of BMJ Publishing Group

RESOURCES