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. Author manuscript; available in PMC: 2023 Aug 16.
Published in final edited form as: Gastroenterology. 2022 Feb 14;162(7):2004–2017.e2. doi: 10.1053/j.gastro.2022.02.017

Figure 5.

Figure 5.

CircANAPC7 suppresses tumor growth in vivo by regulating CREB–miR-373–PHLPP2 feed-forward loop. (A) Cell viability of indicated cells was examined by means of MTT assay. (B) Colony-formation assay. (C) Representative tumor images and tumor weight plot of MIA-ZIP4-EV/circANAPC7 orthotopic models. (D) Representative tumor images and tumor weight plot of AsPC-shZIP4-Pre373-EV/circANAPC7 orthotopic models. (E) Cyclin D1 level was assessed in cell lysates. (F) Cyclin D1 level in orthotopic xenografts. (G, H) Ki67 IHC staining analysis of orthotopic xenografts. (I) Protein levels of PHLPP2, pAKT, and total AKT under 2D and 3D spheroid culture. (J, K) PHLPP2, pCREB, and total CREB expression was assessed by means of Western blot. ns, not significant; *P < .05; **P < .01; ***P < .001.