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. Author manuscript; available in PMC: 2023 Aug 22.
Published in final edited form as: Differentiation. 2022 Sep 24;128:1–12. doi: 10.1016/j.diff.2022.09.002

Fig. 4. SMAD4-I500V leads to transcript expression changes in genes downstream of TGFβ1 and BMP4 signaling.

Fig. 4.

(A) Gene expression of SMAD4, ID3, COL1A1, CTGF, SMAD6 and SMAD7 in untransfected cells treated with or without TGFβ1 or BMP4. (B) Gene expression in cells transfected with WT-SMAD4, SMAD4-I500V or WT/I500V. (C) Gene expression in cells treated with TGFβ1 for 2 hours in cells transfected with WT-SMAD4, SMAD4-I500V, or WT/I500V. (D) Gene expression in cells treated with TGFβ1 for 48 hours in cells transfected with WT-SMAD4, SMAD4-I500V, or WT/I500V. (E) Gene expression in cells treated with BMP4 for 2 hours in cells transfected with WT-SMAD4, SMAD4-I500V, or WT/I500V. (F) Gene expression in cells treated with BMP4 for 48 hours in cells transfected with WT-SMAD4, SMAD4-I500V, or WT/I500V. Differences in expression between WT-SMAD4, SMAD4-I500V, or WT/I500V -transfected cells were statistically analyzed by one-way ANOVA and presented as mean ± SD, ** p < 0.01, *** p < 0.001, **** p < 0.0001; n = 3.