TABLE 1.
Kinetic parameters of enzymes investigated in this study a
| Enzyme | Substrate | kcat | KM | kcat/KM | Assay type |
|---|---|---|---|---|---|
| CpChiAN | 4MU-Chi3 | n.d. | n.d. | n.d. | Chitinase assay kit |
| CpChiAC | 4MU-Chi3 | 159.52 ± 2.1 min−1 | 0.22 ± 0.003 mM | 709.03 | Chitinase assay kit |
| CpChiAC-CBM5 | 4MU-Chi3 | 181.71 ± 2.3 min−1 | 0.29 ± 0.0002 mM | 635.29 | Chitinase assay kit |
| CpChiB | 4MU-Chi3 | 131.94 ± 8.9 min−1 | 0.24 ± 0.01 mM | 530.87 | Chitinase assay kit |
| CpChiB-CBM6 | 4MU-Chi3 | 60.31 ± 3.2 min−1 | 0.12 ± 0.003 mM | 489.12 | Chitinase assay kit |
| CpGlu16A-CBM6 | Curdlan | 2.58 ± 0.2 min−1 | 6.89 ± 1.6 g/L | 0.37 | DNSA |
a
n.d. denotes that no activity was detected. The chitinase activity of CpChiAN was too low to be studied kinetically. Error symbols (±) indicate standard error of the mean.