Skip to main content
. 2023 Aug 17;12(16):2079. doi: 10.3390/cells12162079

Figure 2.

Figure 2

Mycobacterium bovis and its culture filter protein extract (CFPE) promote IL-1β release in bovine macrophages. (A) IL-1β production in bovine macrophages (3 × 105) stimulated with LPS (300 ng/mL) at different times. (B) IL-1β production in macrophages (3 × 105) stimulated with different concentrations of CFPE (25, 50, and 100 µg/mL). (C) IL-1β production in bovine macrophages (3 × 105) stimulated with 100 µg/mL of CFPE/1 × 106 cells at different times. (D) IL-1β production in bovine macrophages (3 × 105) infected with M. bovis AN5 MOI 10:1 at different times. Results are shown as the mean ± S.D. of three independent experiments, each with three internal replicas. One-way ANOVA showed significant differences between the negative control and cells treated at different times or concentrations. * p value ≤ 0.05, ** p value ≤ 0.01, *** p value ≤ 0.001, **** p value ≤ 0.0001.