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. 2023 Aug 17;12(16):2079. doi: 10.3390/cells12162079

Figure 5.

Figure 5

Inhibition of the NLRP3 inflammasome decreased the cell death rate of macrophages. (A,B) LDH in the supernatant of macrophages stimulated with 50 µm Y-VAD, 50 µm Z-VAD, 50 µm Necro-1, 10 µm CRID 3, and Y-VAD plus CRID3, 2 h before stimulation with 100 µg/mL of culture filtrate extract (CFPE) in 1 × 106 cells or M. bovis, for 4 h. (C) LDH in the supernatant of macrophages stimulated with 50 µm Y-VAD, 50 µm Z-VAD, 50 µm Necro-1, 10 µm CRID-3, and Y-VAD + CRID-3. Results are shown as the mean ± S.D. of three independent experiments. One-way ANOVA showed a significant difference between the percentage of cell death in macrophages infected with M. bovis or CFPE versus macrophages infected with M. bovis or CFPE with cell death inhibitors (Y-VAD, Z-VAD, Necro-1, and CRID3). ** p value ≤ 0.01, *** p value ≤ 0.001. H2O2 or hydrogen peroxide a positive control, CFPE, Y-VAD, or Acetyl-tyrosine-valine-alanine-aspartate-chloromethyl ketone an irreversible inhibitor of caspase-1, Z-VAD or acetyl-tyrosine-valine-alanine-aspartate-chloromethyl ketone a pan-caspase inhibitor, Necro-1 or necrostatin-1 inhibitor of RIPK1 and CRID 3 an inhibitor of NLRP3 inflammasome.