Abstract
Two Treponema pallidum haemagglutination assay (TPHA) tests (Wellcome and Fuzizoki) were compared with the automated reagin test and the automated Reiter protein complement-fixation test in a routine screening system. The Wellcome TPHA was shown to lack sensitivity, and it is recommended that it should be used at a lower screening dilution. The Fuzizoki TPHA recorded a significant number of false positive and false negative results, and was therefore inferior to the fluorescent treponemal antibody absorption (FTA-ABS) test as a definitive test for syphilis. It is suggested that the TPHA be used in conjunction with a reagin test to replace some of the more inadequate screening tests currently in use. The automated screening tests were shown to have a similar sensitivity to the TPHA as well as several other advantages; they are recommended for testing large numbers of specimens.
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Selected References
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- Blum G., Ellner P. D., McCarthy L. R., Papachristos T. Reliability of the treponemal hemagglutination test for the serodiagnosis of syphilis. J Infect Dis. 1973 Mar;127(3):321–324. doi: 10.1093/infdis/127.3.321. [DOI] [PubMed] [Google Scholar]
- Coffey E. M., Bradford L. L., Naritomi L. S., Wood R. M. Evaluation of the qualitative and automated quantitative microhemagglutination assay for antibodies to Treponema pallidum. Appl Microbiol. 1972 Jul;24(1):26–30. doi: 10.1128/am.24.1.26-30.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garner M. F., Backhouse J. L., Daskalopoulos G., Walsh J. L. Treponema pallidum haemagglutination test for syphilis. Comparison with TPI and FTA-ABS tests. Br J Vener Dis. 1972 Dec;48(6):470–473. doi: 10.1136/sti.48.6.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnston N. A. Treponema pallidum haemagglutination test for syphilis. Evaluation of a modified micro-method. Br J Vener Dis. 1972 Dec;48(6):474–478. doi: 10.1136/sti.48.6.474. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Le Clair R. A. Evaluation of qualitative hemagglutination test for antibodies to Treponema pallidum. J Infect Dis. 1971 Jun;123(6):668–670. doi: 10.1093/infdis/123.6.668. [DOI] [PubMed] [Google Scholar]
- Macfarlane D. E., Hare K., Elias-Jones T. F. Evaluation of automated large-scale screening tests for syphilis. J Clin Pathol. 1976 Apr;29(4):317–321. doi: 10.1136/jcp.29.4.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Neill P., Warner R. W., Nicol C. S. Treponema pallidum haemagglutination assay in the routine serodiagnosis of treponemal disease. Br J Vener Dis. 1973 Oct;49(5):427–431. doi: 10.1136/sti.49.5.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sequeira P. J., Eldridge A. E. Treponemal haemagglutination test. Br J Vener Dis. 1973 Jun;49(3):242–248. doi: 10.1136/sti.49.3.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tomizawa T. Hemagglutination tests for diagnosis of syphilis. A preliminary report. Jpn J Med Sci Biol. 1966 Dec;19(6):305–308. doi: 10.7883/yoken1952.19.305. [DOI] [PubMed] [Google Scholar]