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. 1979 Apr;55(2):90–93. doi: 10.1136/sti.55.2.90

Preservation of Neisseria gonorrhoeae by the gelatin-disc method.

S Yamai, Y Obara, T Nikkawa, Y Shimoda, Y Miyamoto
PMCID: PMC1045596  PMID: 109165

Abstract

Studies of Neisseria gonorrhoeae are difficult to perform because of the organism's poor survival in vitro. To solve this problem we tried to preserve the organism by a gelatin-disc method. The rate of survival and changes of variations in some biochemical properties of eight strains of N. gonorrhoeae were followed for three years. These studies proved that preservation was satisfactory with only a 1/10 reduction of the living cells. Another trial showed that the organism survived for over six months after being frozen at -20 degrees C. The colonial types, agglutination against red cells from rabbit and guinea pig, and antibiotic susceptibility to penicillin, chloramphenicol, tetracycline, kanamycin, and streptomycin did not change after three years' preservation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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