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. 1979 Aug;55(4):239–244. doi: 10.1136/sti.55.4.239

Evaluation of microhaemagglutination assay to determine treponemal antibodies in CSF.

A Birry, S Kasatiya
PMCID: PMC1045647  PMID: 385012

Abstract

The microhaemagglutination assay for Treponema pallidum antibodies (MHA:TP-CSF), the Veneral Disease Research Laboratory (VDRL-CSF), and the fluorescent treponemal antibody (FTA-CSF) tests were used to detect treponemal antibodies in 3157 samples of cerebrospinal fluid from patients with syphilis, with neurological, metabolic, or immunological disorders, and with undetermined diagnoses. An agreement of 99.5% between the results of the MHA:TP-CSF and FTA-CSF tests was obtained for all the CSF samples whereas that of only 95.5% was obtained for samples from the known case of syphilis. The MHA:TP-CSF test is simple, economical, rapid, and specific but not as sensitive as the FTA-CSF test.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coffey E. M., Bradford L. L., Naritomi L. S., Wood R. M. Evaluation of the qualitative and automated quantitative microhemagglutination assay for antibodies to Treponema pallidum. Appl Microbiol. 1972 Jul;24(1):26–30. doi: 10.1128/am.24.1.26-30.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Duncan W. P., Jenkins T. W., Parham C. E. Fluorescent treponemal antibody-cerebrospinal fluid (FTA-CSF) test. A provisional technique. Br J Vener Dis. 1972 Apr;48(2):97–101. doi: 10.1136/sti.48.2.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Garner M. F., Backhouse J. L., Daskalopoulos G., Walsh J. L. The Treponema pallidum haemagglutination (TPHA) test in biological false positive and leprosy sera. J Clin Pathol. 1973 Apr;26(4):258–260. doi: 10.1136/jcp.26.4.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Garner M. F., Backhouse J. L., Daskalopoulos G., Walsh J. L. Treponema pallidum haemagglutination test for syphilis. Comparison with TPI and FTA-ABS tests. Br J Vener Dis. 1972 Dec;48(6):470–473. doi: 10.1136/sti.48.6.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jaffe H. W., Larsen S. A., Peters M., Jove D. F., Lopez B., Schroeter A. L. Tests for treponemal antibody in CSF. Arch Intern Med. 1978 Feb;138(2):252–255. [PubMed] [Google Scholar]
  6. Kasatiya S. S., Birry A. The use of incident light in immunofluorescence applied to syphilis serology. Am J Clin Pathol. 1972 Mar;57(3):395–399. doi: 10.1093/ajcp/57.3.395. [DOI] [PubMed] [Google Scholar]
  7. Király K., Prerau H. Evaluation of the T. pallidum haemagglutination (TPHA) test for syphilis on "problem sera". Acta Derm Venereol. 1974;54(4):303–310. [PubMed] [Google Scholar]
  8. Kopecký K., Chlíková M., Fadrhoncova A. A microhaemagllutination test in the diagnostics of syphilis. J Hyg Epidemiol Microbiol Immunol. 1977;21(1):23–32. [PubMed] [Google Scholar]
  9. Leclerc G., Giroux M., Birry A., Kasatiya S. Study of fluorescent treponemal antibody test on cerebrospinal fluid using monospecific anti-immunoglobulin conjugates IgG, IgM, and IgA. Br J Vener Dis. 1978 Oct;54(5):303–308. [PMC free article] [PubMed] [Google Scholar]
  10. Lesiński J., Krach J., Kadziewicz E. Specificity, sensitivity, and diagnostic value of the TPHA test. Br J Vener Dis. 1974 Oct;50(5):334–340. doi: 10.1136/sti.50.5.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Luger A., Spendlingwimmer I. Appraisal of the Treponema pallidum haemagglutination test. Br J Vener Dis. 1973 Apr;49(2):181–182. doi: 10.1136/sti.49.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Monsiorska-Borowska Z. Badania płynów mózgowo-rdzeniowych odczynem TPHA w kile pónej. Przegl Dermatol. 1977 Nov-Dec;64(6):705–710. [PubMed] [Google Scholar]
  13. O'Neill P., Warner R. W., Nicol C. S. Treponema pallidum haemagglutination assay in the routine serodiagnosis of treponemal disease. Br J Vener Dis. 1973 Oct;49(5):427–431. doi: 10.1136/sti.49.5.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Oxelius V. A., Rorsman H., Laurell A. B. Immunoglobulins of cerebrospinal fluid in syphilis. Br J Vener Dis. 1969 Jun;45(2):121–125. doi: 10.1136/sti.45.2.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rathlev T. Haemagglutination test utilizing pathogenic Treponema pallidum for the sero-diagnosis of syphilis. Br J Vener Dis. 1967 Sep;43(3):181–185. doi: 10.1136/sti.43.3.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rudolph A. H. The microhemagglutination assay for Treponema pallidum antibodies (MHA-TP), a new treponemal test for syphilis: where does it fit? J Am Vener Dis Assoc. 1976 Sep;3(1):3–8. [PubMed] [Google Scholar]
  17. Sequeira P. J., Eldridge A. E. Treponemal haemagglutination test. Br J Vener Dis. 1973 Jun;49(3):242–248. doi: 10.1136/sti.49.3.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tomizawa T., Kasamatsu S., Yamaya S. I. Usefulness of the hemagglutination test using Treponema pallidum antigen (TPHA) for the serodiagnosis of syphilis. Jpn J Med Sci Biol. 1969 Dec;22(6):341–350. doi: 10.7883/yoken1952.22.341. [DOI] [PubMed] [Google Scholar]
  19. Young H., Henrichsen C., Robertson D. H. Treponema pallidum haemagglutination test as a screening procedure for the diagnosis of syphilis. Br J Vener Dis. 1974 Oct;50(5):341–346. doi: 10.1136/sti.50.5.341. [DOI] [PMC free article] [PubMed] [Google Scholar]

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