Abstract
Quantative determinations of the mucociliary activity of human Fallopian tube epithelium maintained as organ cultures were performed using a light beam reflex method. In non-infected organ cultures the mucociliary wave (MCW) frequency slowly decreased during the first 54 hours of culture maintenance. In organ cultures experimentally infected with fresh isolates of Neisseria gonorrhoeae producing T1/T2 colonies the MCW frequency either decreased to subnormal values or completely ceased whereas in organ cultured infected with a laboratory-adapted gonococcal strain the MCW frequencies remained within normal range. In organ cultures exposed to gonococcal endotoxin prepared from the laboratory-adapted strain, as well as in cultures in which cell-free filtrates of medium from organ cultures infected with N. gonorrhoeae (producing T1/T2 colonies) were added to the culture medium, the ciliary activity decreased and subsequently ceased. The same phenomenon occurred when organ cultures were challenged with Escherichia coli endotoxin. The ciliostatic effect appeared before any morphological changes in the surface epithelium, including the cilia, were demonstrable by scanning electron microscopy.
Full text
PDF








Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Carney F. E., Jr, Taylor-Robinson D. Growth and effect of Neisseria gonorrhoeae in organ cultures. Br J Vener Dis. 1973 Oct;49(5):435–440. doi: 10.1136/sti.49.5.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hakansson C. H., Toremalm N. G. Studies on the physiology of the trachea. I. Ciliary activity indirectly recorded by a new "light beam reflex" method. Ann Otol Rhinol Laryngol. 1965 Dec;74(4):954–969. doi: 10.1177/000348946507400404. [DOI] [PubMed] [Google Scholar]
- KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maeland J. A. Antigenic properties of various preparations of Neisseria gonorrhoeae endotoxin. Acta Pathol Microbiol Scand. 1968;73(3):413–422. doi: 10.1111/j.1699-0463.1968.tb04610.x. [DOI] [PubMed] [Google Scholar]
- McGee Z. A., Johnson A. P., Taylor-Robinson D. Human fallopian tubes in organ culture: preparation, maintenance, and quantitation of damage by pathogenic microorganisms. Infect Immun. 1976 Feb;13(2):608–618. doi: 10.1128/iai.13.2.608-618.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mårdh P. A., Weström L., von Mecklenburg C., Hammar E. Studies on ciliated epithelia of the human genital tract. I. Swelling of the cilia of Fallopian tube epithelium in organ cultures infected with Mycoplasma hominis. Br J Vener Dis. 1976 Feb;52(1):52–57. doi: 10.1136/sti.52.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rees E., Annels E. H. Gonococcal salpingitis. Br J Vener Dis. 1969 Sep;45(3):205–215. doi: 10.1136/sti.45.3.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor-Robinson D., Whytock S., Green C. J., Carney F. E., Jr Effect of Neisseria gonorrhoeae on human and rabbit oviducts. Br J Vener Dis. 1974 Aug;50(4):279–288. doi: 10.1136/sti.50.4.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Toremalm N. G., Håkansson C. H., Mercke U., Dahlerus B. Mucociliary wave pattern. An analysis of surface light reflections. Acta Otolaryngol. 1974 Sep-Oct;78(3-4):247–252. doi: 10.3109/00016487409126351. [DOI] [PubMed] [Google Scholar]
- Ward M. E., Watt P. J., Robertson J. N. The human fallopian tube: a laboratory model for gonococcal infection. J Infect Dis. 1974 Jun;129(6):650–659. doi: 10.1093/infdis/129.6.650. [DOI] [PubMed] [Google Scholar]
- Weström L., Mårdh P. A., Mecklenburg C. V., Håkansson C. H. Studies on ciliated epithelia of the human genital tract. II. The mucociliary wave pattern of fallopian tube epithelium. Fertil Steril. 1977 Sep;28(9):955–961. doi: 10.1016/s0015-0282(16)42798-6. [DOI] [PubMed] [Google Scholar]