Figure 2.

Set-up of the optimal experimental condition for the delivery of a naked PNA mediated by calix[4]arene vectors. (A) Histograms indicating the percentage of fluorescent positive cells, detected by FACS analysis in the absence (−) or presence (+) of the Arg-Hex vector, versus the increasing concentrations (0.5–2 µM) of carboxyfluorescein-labelled naked PNA. Results are presented as mean ± S.E.M; statistical differences between groups were compared using a paired t-test ANOVA. (*): p < 0.05 (significant); (**): p < 0.01 (highly significant). (B) FACS analysis plot: overlay of naked-PNA complexed with Arg-Hex (red), vector: Arg-Hex (green), naked PNA (blue).