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. 1982 Jun;58(3):166–175. doi: 10.1136/sti.58.3.166

Auxotypes and antibacterial resistance to gonococci with differing susceptibilities to vancomycin.

A C Exner, E N Shinners, P J Pace, B W Catlin
PMCID: PMC1046038  PMID: 6805849

Abstract

The responses to vancomycin and 11 other antibacterial drugs and the nutritional requirements of gonococci recovered from two selective media were determined. Single urogenital specimens from 508 patients attending a social hygiene clinic in 1975 yielded 97 strains of Neisseria gonorrhoeae; 95 were recovered on VCNT (a modification of Thayer-Martin medium), always inoculated first, and 69 on LC medium containing lincomycin (4 micrograms/ml) and colistin (5 micrograms/ml). The two drugs at these concentrations in LC medium were not inhibitory for isolates from either medium. Unexpectedly, three isolates on VCNT were susceptible to vancomycin at the concentrations (3 micrograms/ml) in VCNT medium; these three were typically sensitive to penicillins but were hypersusceptible to erythromycin (inhibited by less than or greater than 0.05 micrograms/ml) and rifampin (less than or equal to 0.02 micrograms/ml). Resistance to streptomycin (greater than or equal to 500 micrograms/ml) (22% of the strains) was correlated with increased resistance to penicillins, erythromycin, and rifampin in most instances. All streptomycin-resistant gonococci required proline, or arginine, or none of the test compounds. Strains requiring arginine, hypoxanthine, and uracil were uniformly sensitive to antibiotics but not hypersusceptible. In contrast, six strains of N gonorrhoeae isolated in Denmark required arginine (not satisfied by ornithine), hypoxanthine, and uracil and were hypersusceptible to vancomycin (inhibited by 0.5 micrograms/ml), erythromycin, and rifampin. DNA-mediated transformation showed that all three hypersusceptibilities of one Danish strain were introduced together into a wild-type gonococcus, suggesting that a mutation of an env (envelope) locus might be responsible for the atypical permeability.

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Selected References

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  1. Brorson J. E., Holmberg I., Nygren B., Seeberg S. Vancomycin-sensitive strains of Neisseria gonorrhoeae. A problem for the diagnostic laboratory. Br J Vener Dis. 1973 Oct;49(5):452–453. doi: 10.1136/sti.49.5.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Catlin B. W. Genetic transformation of biosynthetically defective Neisseria gonorrhoeae clinical isolates. J Bacteriol. 1974 Oct;120(1):203–209. doi: 10.1128/jb.120.1.203-209.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Catlin B. W., Pace P. J. Auxotypes and penicillin susceptibilities of Neisseria gonorrhoeae isolated from patients with gonorrhea involving two or more sites. Antimicrob Agents Chemother. 1977 Aug;12(2):147–156. doi: 10.1128/aac.12.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Catlin B. W., Pace P. J. Nutritional requirements and penicillin susceptibilities of gonococci from pharyngeal and anogenital sites. Br J Vener Dis. 1977 Oct;53(5):299–303. doi: 10.1136/sti.53.5.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Catlin B. W., Reyn A. Neisseria gonorrhoeae isolated from disseminated and localised infections in pre-penicillin era. Auxotypes and antibacterial drug resistances. Br J Vener Dis. 1982 Jun;58(3):158–165. doi: 10.1136/sti.58.3.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cross R. C., Hoger M. B., Neibaur R., Pasternack B., Brady F. J. VCN--inhibited strains of Neisseria gonorrhoeae. HSMHA Health Rep. 1971 Nov;86(11):990–992. [PMC free article] [PubMed] [Google Scholar]
  8. Eisenstein B. I., Sparling P. F. Mutations to increased antibiotic sensitivity in naturally-occurring gonococci. Nature. 1978 Jan 19;271(5642):242–244. doi: 10.1038/271242a0. [DOI] [PubMed] [Google Scholar]
  9. Evans A. J., Morrison G. D., Price D. J. Prolonged use of the Greenland method of treatment of gonorrhoea. Br J Vener Dis. 1980 Apr;56(2):88–91. doi: 10.1136/sti.56.2.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Faur Y. C., Weisburd M. H., Wilson M. E. The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrhoeae from clinical specimens. Health Lab Sci. 1978 Jan;15(1):22–27. [PubMed] [Google Scholar]
  11. GOCKE T. M., WILCOX C., FINLAND M. Antibiotic spectrum of the gonococcus. Am J Syph Gonorrhea Vener Dis. 1950 May;34(3):265–272. [PubMed] [Google Scholar]
  12. Guymon L. F., Walstad D. L., Sparling P. F. Cell envelope alterations in antibiotic-sensitive and-resistant strains of Neisseria gonorrhoeae. J Bacteriol. 1978 Oct;136(1):391–401. doi: 10.1128/jb.136.1.391-401.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jaffe H. W., Biddle J. W., Thornsberry C., Johnson R. E., Kaufman R. E., Reynolds G. H., Wiesner P. J. National gonorrhea therapy monitoring study: in vitro antibiotic susceptibility and its correlation with treatment results. N Engl J Med. 1976 Jan 1;294(1):5–9. doi: 10.1056/NEJM197601012940102. [DOI] [PubMed] [Google Scholar]
  14. Knapp J. S., Thornsberry C., Schoolnik G. A., Wiesner P. J., Homes K. K. Phenotypic and epidemiologic correlates of auxotype in Neisseria gonorrhoeae. J Infect Dis. 1978 Aug;138(2):160–165. doi: 10.1093/infdis/138.2.160. [DOI] [PubMed] [Google Scholar]
  15. Le Faou A., Guy I., Riou J. Y. Auxotypes et sensibilité a 6 antibiotiques des souches de Neisseria gonorrhoeae isolées a Strasbourg en 1977-1978. Ann Dermatol Venereol. 1979 Mar;106(3):267–272. [PubMed] [Google Scholar]
  16. Martin J. E., Jr, Lester A., Price E. V., Schmale J. D. Comparative study of gonococcal susceptibility to penicillin in the United States, 1955-1969. J Infect Dis. 1970 Nov;122(5):459–461. doi: 10.1093/infdis/122.5.459. [DOI] [PubMed] [Google Scholar]
  17. Meheus A., Piot P., Pattyn S., van Dyck E., van den Berghe D. Activity in vitro of ten antimicrobial agents against Neisseria gonorrhoeae. A study of the correlation between the sensitivities. Br J Vener Dis. 1976 Oct;52(5):329–332. doi: 10.1136/sti.52.5.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mirrett S., Reller L. B., Knapp J. S. Neisseria gonorrhoeae strains inhibited by vancomycin in selective media and correlation with auxotype. J Clin Microbiol. 1981 Jul;14(1):94–99. doi: 10.1128/jcm.14.1.94-99.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Noble R. C. Characterisation of Neisseria gonorrhoeae from women with simultaneous infections at two sites. Br J Vener Dis. 1980 Feb;56(1):3–5. doi: 10.1136/sti.56.1.3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Odegaard K., Solberg O., Lind J., Myhre G., Nyland B. Lincomycin in selective medium for the isolation of Neisseria gonorrhoeae. Acta Pathol Microbiol Scand B. 1975 Jun;83(3):301–304. doi: 10.1111/j.1699-0463.1975.tb00106.x. [DOI] [PubMed] [Google Scholar]
  21. Phillips I., Humphrey D., Middleton A., Nicol C. S. Diagnosis of gonorrhoea by culture on a selective medium containing vancomycin, colistin, nystatin and trimethoprim (VCNT). A comparison with Gram-staining and immunofluorescence. Br J Vener Dis. 1972 Aug;48(4):287–292. doi: 10.1136/sti.48.4.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Phillips I., Ridley M., Rimmer D., Lynn R. In-vitro activity of twelve antibacterial agents against Neisseria gonorrhoeae. Lancet. 1970 Feb 7;1(7641):263–265. doi: 10.1016/s0140-6736(70)90635-5. [DOI] [PubMed] [Google Scholar]
  23. Platt D. J., Gerken A. Inhibition of gonococci by a selective medium: disparity between isolates from sexual partners. Br J Vener Dis. 1979 Feb;55(1):65–65. doi: 10.1136/sti.55.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Platt D. J. Prevalence of multiple antibiotic resistance in Neisseria gonorrhoeae. Br J Vener Dis. 1976 Dec;52(6):384–386. doi: 10.1136/sti.52.6.384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. REYN A., BENTZON M. W., THAYER J. D., WILKINSON A. E. RESULTS OF COMPARATIVE EXPERIMENTS USING DIFFERENT METHODS FOR DETERMINING THE SENSITIVITY OF NEISSERIA GONORRHOEAE TO PENICILLIN G. Bull World Health Organ. 1965;32:477–502. [PMC free article] [PubMed] [Google Scholar]
  26. REYN A., KORNER B., BENTZON M. W. Effects of penicillin, streptomycin, and tetracycline on N. gonorrhoeae isolated in 1944 and in 1957. Br J Vener Dis. 1958 Dec;34(4):227–239. doi: 10.1136/sti.34.4.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. REYN A. Sensitivity of N. gonorrhoeae to antibiotics. Br J Vener Dis. 1961 Jun;37:145–157. doi: 10.1136/sti.37.2.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Reyn A., Bentzon M. W. Comparison of a selective and a non-selective medium in the diagnosis of gonorrhoea to ascertain the sensitivity of Neisseria gonorrhoeae to vancomycin. Br J Vener Dis. 1972 Oct;48(5):363–368. doi: 10.1136/sti.48.5.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Reyn A., Benzon M. W. A study of the relationships between the sensitivities of Neisseria gonorrhoeae to sodium penicillin G, four semi-synthetic penicillins, spiramycin, and fusidic acid. Br J Vener Dis. 1968 Jun;44(2):140–150. doi: 10.1136/sti.44.2.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Reyn A. Recent developments in the laboratory diagnosis of gonococcal infections. Bull World Health Organ. 1969;40(2):245–255. [PMC free article] [PubMed] [Google Scholar]
  31. Seth A. D., Kolator B., Wilkinson A. E. Sensitivity of Neisseria gonorrhoeae to antibiotics in London (1976-78). Br J Vener Dis. 1979 Oct;55(5):325–328. doi: 10.1136/sti.55.5.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Stewart I. O., Hendry A. T. Association between the auxogroup of Neisseria gonorrhoeae and the minimal inhibitory concentration of penicillin. Sex Transm Dis. 1979 Oct-Dec;6(4):247–252. doi: 10.1097/00007435-197910000-00004. [DOI] [PubMed] [Google Scholar]
  33. Taylor E., Phillips I. Assessment of a selective medium for the isolation of Neisseria gonorrhoeae. Br J Vener Dis. 1979 Jun;55(3):183–185. doi: 10.1136/sti.55.3.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Thayer J. D., Martin J. E., Jr Improved medium selective for cultivation of N. gonorrhoeae and N. meningitidis. Public Health Rep. 1966 Jun;81(6):559–562. [PMC free article] [PubMed] [Google Scholar]
  35. Warner P. F., Zubrzycki L. J., Chila M. Polygenes and modifier genes for tetracycline and penicillin resistance in Neisseria gonorrhoeae. J Gen Microbiol. 1980 Mar;117(1):103–110. doi: 10.1099/00221287-117-1-103. [DOI] [PubMed] [Google Scholar]
  36. Willcox R. R. A survey of problems in the antibiotic treatment of gonorrhoea. With special reference to South-East Asia. Br J Vener Dis. 1970 Jun;46(3):217–242. doi: 10.1136/sti.46.3.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Windall J. J., Hall M. M., Washington J. A., 2nd, Douglass T. J., Weed L. A. Inhibitory effects of vancomycin on Neisseria gonorrhoeae in Thayer-Martin medium. J Infect Dis. 1980 Nov;142(5):775–775. doi: 10.1093/infdis/142.5.775. [DOI] [PubMed] [Google Scholar]

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