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. 1982 Aug;58(4):211–219. doi: 10.1136/sti.58.4.211

Scanning electron microscopy of the attachment of Treponema pallidum to nerve cells in vitro.

L A Repesh, T J Fitzgerald, S G Oakes, R S Pozos
PMCID: PMC1046052  PMID: 7049315

Abstract

Treponema pallidum (Nichols strain) was incubated with cultured nerve cells derived from rat embryos. Primary cultures were established from dorsal root ganglia, superior cervical ganglia, and spinal cord. Using phase contrast microscopy treponemes were seen to interact with the nerve cells in a similar manner to other cultured mammalian cells. Organisms began to attach within minutes after inoculation, actively motile organisms attached at the tip of one end, higher numbers of organisms attached with continued incubation, and attached organisms survived longer than unattached organisms. T pallidum attached both to nerve cell bodies and to neuronal processes of each of the three nerve cell cultures. As shown by scanning electron microscopy the mechanism of attachment was identical to that of cultured cells derived from rabbits testis, rat skeletal muscle, and human cervical carcinoma. There was no indentation or swelling of the cultured cell surface at the point of attachment, just a close physical proximity of organisms and cells. These techniques provide a biological means of studying the in-vitro detrimental influences of micro-organisms on nerve tissue.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fieldsteel A. H., Becker F. A., Stout J. G. Prolonged survival of virulent Treponema pallidum (Nichols strain) in cell-free and tissue culture systems. Infect Immun. 1977 Oct;18(1):173–182. doi: 10.1128/iai.18.1.173-182.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fitzgerald T. J., Cleveland P., Johnson R. C., Miller J. N., Sykes J. A. Scanning electron microscopy of Treponema pallidum (Nichols strain) attached to cultured mammalian cells. J Bacteriol. 1977 Jun;130(3):1333–1344. doi: 10.1128/jb.130.3.1333-1344.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fitzgerald T. J., Johnson R. C., Miller J. N., Sykes J. A. Characterization of the attachment of Treponema pallidum (Nichols strain) to cultured mammalian cells and the potential relationship of attachment to pathogenicity. Infect Immun. 1977 Nov;18(2):467–478. doi: 10.1128/iai.18.2.467-478.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fitzgerald T. J., Miller J. N., Sykes J. A. Treponema pallidum (Nichols strain) in tissue cultures: cellular attachment, entry, and survival. Infect Immun. 1975 May;11(5):1133–1140. doi: 10.1128/iai.11.5.1133-1140.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fitzgerald T. J. Pathogenesis and immunology of Treponema pallidum. Annu Rev Microbiol. 1981;35:29–54. doi: 10.1146/annurev.mi.35.100181.000333. [DOI] [PubMed] [Google Scholar]
  6. Fitzgerald T. J., Repesh L. A., Oakes S. G. Morphological destruction of cultured cells by the attachment of Treponema pallidum. Br J Vener Dis. 1982 Feb;58(1):1–11. doi: 10.1136/sti.58.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hayes N. S., Muse K. E., Collier A. M., Baseman J. B. Parasitism by virulent Treponema pallidum of host cell surfaces. Infect Immun. 1977 Jul;17(1):174–186. doi: 10.1128/iai.17.1.174-186.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Király K., Horváth I. Survival of T. pallidum under microaerobic conditions in cell and tissue cultures. Zentralbl Bakteriol Orig A. 1976 Aug;235(4):500–505. [PubMed] [Google Scholar]
  9. Oakes S. G., Petry R. W., Ziegler R. J., Pozos R. S. Electrophysiological changes of HSV-1-infected dorsal root ganglia neurons in culture. J Neuropathol Exp Neurol. 1981 Jul;40(4):380–389. doi: 10.1097/00005072-198107000-00003. [DOI] [PubMed] [Google Scholar]
  10. Ovcinnikov N. M., Delektorskij V. V. Treponema pallidum in nerve fibres. Br J Vener Dis. 1975 Feb;51(1):10–18. doi: 10.1136/sti.51.1.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ransom B. R., Neale E., Henkart M., Bullock P. N., Nelson P. G. Mouse spinal cord in cell culture. I. Morphology and intrinsic neuronal electrophysiologic properties. J Neurophysiol. 1977 Sep;40(5):1132–1150. doi: 10.1152/jn.1977.40.5.1132. [DOI] [PubMed] [Google Scholar]
  12. Sandok P. L., Jenkin H. M., Graves S. R., Knight S. T. Retention of motility of Treponema pallidum (Nichols virulent strain) in an anaerobic cell culture system and in a cell-free system. J Clin Microbiol. 1976 Jan;3(1):72–74. doi: 10.1128/jcm.3.1.72-74.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wrzolkowa T., Kozakiewicz J. Ultrastructure of vascular and connective tissue changes in primary syphilis. Br J Vener Dis. 1980 Jun;56(3):137–143. doi: 10.1136/sti.56.3.137. [DOI] [PMC free article] [PubMed] [Google Scholar]

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