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. 1983 Feb;59(1):21–29. doi: 10.1136/sti.59.1.21

Factors affecting the attachment of Treponema pallidum to mammalian cells in vitro.

G H Wong, B Steiner, S Faine, S Graves
PMCID: PMC1046124  PMID: 6337680

Abstract

Attachment of Treponema pallidum (Nichols) to mammalian cells is probably the first step in the pathogenesis of syphilis. It may also be important for the multiplication of T pallidum in vitro. When factors affecting the attachment of T pallidum to mammalian cells in vitro were studied significantly greater numbers of treponemes were found to attach to baby rabbit genital organ (BRGO) cells than to five other mammalian cell lines. When attached to BRGO cells T pallidum survived longer in vitro than unattached treponemes. Eagle's minimal essential medium was superior to three other culture media in increasing attachment and maintaining the survival of treponemes. Dithiothreitol (0.25-1.0 mmol/l) had no effect on the attachment of T pallidum to BRGO cells. Anaerobic conditions were superior to microaerophilic conditions, and the latter were superior to aerobic conditions for the attachment and survival of T pallidum to BRGO cells. Within the range of concentrations tested the number of treponemes attached to the BRGO cells was directly dependent on the concentrations of viable treponemes in the inoculum. Greater numbers of treponemes attached to actively metabolising BRGO cells than to quiescent or slowly growing cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alderete J. F., Baseman J. B. Analysis of serum IgG against Treponema pallidum protein antigens in experimentally infected rabbits. Br J Vener Dis. 1981 Oct;57(5):302–308. doi: 10.1136/sti.57.5.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baseman J. B., Hayes E. C. Molecular characterization of receptor binding proteins and immunogens of virulent Treponema pallidum. J Exp Med. 1980 Mar 1;151(3):573–586. doi: 10.1084/jem.151.3.573. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baseman J. B., Nichols J. C., Hayes N. C. Virulent Treponema pallidum: aerobe or anaerobe. Infect Immun. 1976 Mar;13(3):704–711. doi: 10.1128/iai.13.3.704-711.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chalmers W. S., Taylor-Robinson D. The effect of reducing and other agents on the motility of Treponema pallidum in an acellular culture medium. J Gen Microbiol. 1979 Oct;114(2):443–447. doi: 10.1099/00221287-114-2-443. [DOI] [PubMed] [Google Scholar]
  5. Fieldsteel A. H., Cox D. L., Moeckli R. A. Cultivation of virulent Treponema pallidum in tissue culture. Infect Immun. 1981 May;32(2):908–915. doi: 10.1128/iai.32.2.908-915.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fieldsteel A. H., Stout J. G., Becker F. A. Comparative behavior of virulent strains of Treponema pallidum and Treponema pertenue in gradient cultures of various mammalian cells. Infect Immun. 1979 May;24(2):337–345. doi: 10.1128/iai.24.2.337-345.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fitzgerald T. J., Johnson R. C., Miller J. N., Sykes J. A. Characterization of the attachment of Treponema pallidum (Nichols strain) to cultured mammalian cells and the potential relationship of attachment to pathogenicity. Infect Immun. 1977 Nov;18(2):467–478. doi: 10.1128/iai.18.2.467-478.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fitzgerald T. J., Johnson R. C. Mucopolysaccharidase of Treponema pallidum. Infect Immun. 1979 Apr;24(1):261–268. doi: 10.1128/iai.24.1.261-268.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fitzgerald T. J., Johnson R. C., Wolff E. T. Sulfhydryl oxidation using procedures and experimental conditions commonly used for Treponema pallidum. Br J Vener Dis. 1980 Jun;56(3):129–136. doi: 10.1136/sti.56.3.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fitzgerald T. J. Pathogenesis and immunology of Treponema pallidum. Annu Rev Microbiol. 1981;35:29–54. doi: 10.1146/annurev.mi.35.100181.000333. [DOI] [PubMed] [Google Scholar]
  11. Graves S., Billington T. Optimum concentration of dissolved oxygen for the survival of virulent Treponema pallidum under conditions of low oxidation-reduction potential. Br J Vener Dis. 1979 Dec;55(6):387–393. doi: 10.1136/sti.55.6.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hayes N. S., Muse K. E., Collier A. M., Baseman J. B. Parasitism by virulent Treponema pallidum of host cell surfaces. Infect Immun. 1977 Jul;17(1):174–186. doi: 10.1128/iai.17.1.174-186.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Horváth I., Duncan W. P., Bullard J. C. Cultivation of pathogenic Treponema pallidum in vitro. Acta Microbiol Acad Sci Hung. 1981;28(1):7–24. [PubMed] [Google Scholar]
  14. Király K., Horváth I. Survival of T. pallidum under microaerobic conditions in cell and tissue cultures. Zentralbl Bakteriol Orig A. 1976 Aug;235(4):500–505. [PubMed] [Google Scholar]
  15. Peterkofsky B., Prather W. Cytotoxicity of ascorbate and other reducing agents towards cultured fibroblasts as a result of hydrogen peroxide formation. J Cell Physiol. 1977 Jan;90(1):61–70. doi: 10.1002/jcp.1040900109. [DOI] [PubMed] [Google Scholar]
  16. Rathlev T. Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Br J Vener Dis. 1975 Oct;51(5):296–300. doi: 10.1136/sti.51.5.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sandok P. L., Jenkin H. M., Matthews H. M., Roberts M. S. Unsustained multiplication of treponema pallidum (nichols virulent strain) in vitro in the presence of oxygen. Infect Immun. 1978 Feb;19(2):421–429. doi: 10.1128/iai.19.2.421-429.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Steiner B., McLean I., Graves S. Redox potential and survival of virulent Treponema pallidum under microaerophilic conditions. Br J Vener Dis. 1981 Oct;57(5):295–301. doi: 10.1136/sti.57.5.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wong G. H., Steiner B. M., Graves S. R. Effects of anaerobic and microaerophilic conditions of extraction and incubation on the survival of Treponema pallidum in vitro. Br J Vener Dis. 1982 Jun;58(3):139–142. doi: 10.1136/sti.58.3.139. [DOI] [PMC free article] [PubMed] [Google Scholar]

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