Extended Data Fig. 1 |. Cryo-EM data processing and validation.
a-c, Image processing and map validation of MRS2Mg (a), MRS2EDTA (b), and MRS2Ca (c). Representative micrographs and 2D classes are shown. The final 3D reconstructions are colored by local resolution. Also shown are Fourier shell correlations (FSC) and orientation distribution of particles used in the final reconstruction.