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. 1983 Dec;59(6):349–358. doi: 10.1136/sti.59.6.349

Studies of rabbit testes infected with Treponema pallidum. I Immunopathology.

K Wicher, V Wicher, S M Nakeeb, S Dubiski
PMCID: PMC1046236  PMID: 6357348

Abstract

Rabbit testes were injected with suspensions of Treponema pallidum, washed T pallidum, heat killed T pallidum, or Reiter treponemes. The testes were removed three to 24 days after injection and examined for the number of treponemes, the presence of treponemal antibodies, histopathological changes, and presence of T and B cells. In animals infected with T pallidum a substantial number (10(6)-10(7)/ml) of organisms were still present at day 24 in spite of early local production of antibodies and increasing infiltration with plasma cells, T lymphocytes, and macrophages. In animals infected with washed T pallidum a lower degree of inflammation was observed than in those infected with unmodified T pallidum, and the treponemal antibodies were detected simultaneously in samples of testicular fluid and serum. In the groups injected with heat killed T pallidum and Reiter treponemes no macroscopical or microscopical changes were detected, although in the group injected with heat killed T pallidum treponemal antibodies were detected in the testicular fluid on day 24.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azar H. A., Pham T. D., Kurban A. K. An electron microscopic study of a syphilitic chancre. Engulfment of Treponema pallidum by plasma cells. Arch Pathol. 1970 Aug;90(2):143–150. [PubMed] [Google Scholar]
  2. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  3. CHRISTIANSEN S. Protective layer covering pathogenic treponemata. Lancet. 1963 Feb 23;1(7278):423–425. doi: 10.1016/s0140-6736(63)92309-2. [DOI] [PubMed] [Google Scholar]
  4. Dubiski S. Immunochemistry and genetics of a "new" allotypic specificity Ae14 of rabbit gamma-G immunoglobulins: recombination in somatic cells. J Immunol. 1969 Jul;103(1):120–128. [PubMed] [Google Scholar]
  5. HANSON A. W., CANNEFAX G. R. RECOVERY OF TREPONEMA AND BORRELIA AFTER LYOPHILIZATION. J Bacteriol. 1964 Sep;88:811–811. doi: 10.1128/jb.88.3.811-811.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hardy P. H., Jr, Graham D. J., Nell E. E., Dannenberg A. M., Jr Macrophages in immunity to syphilis: suppressive effect of concurrent infection with Mycobacterium bovis BCG on the development of syphilitic lesions and growth of Treponema pallidum in tuberculin-positive rabbits. Infect Immun. 1979 Nov;26(2):751–763. doi: 10.1128/iai.26.2.751-763.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lukehart S. A., Baker-Zander S. A., Lloyd R. M., Sell S. Characterization of lymphocyte responsiveness in early experimental syphilis. II. Nature of cellular infiltration and Treponema pallidum distribution in testicular lesions. J Immunol. 1980 Jan;124(1):461–467. [PubMed] [Google Scholar]
  8. Lukehart S. A., Baker-Zander S. A., Sell S. Characterization of lymphocyte responsiveness in early experimental syphilis. I. In vitro response to mitogens and Treponema pallidum antigens. J Immunol. 1980 Jan;124(1):454–460. [PubMed] [Google Scholar]
  9. Penn C. W. Avoidance of host defences by Treponema pallidum in situ and on extraction from infected rabbit testes. J Gen Microbiol. 1981 Sep;126(1):69–75. doi: 10.1099/00221287-126-1-69. [DOI] [PubMed] [Google Scholar]
  10. SCOTT V., DAMMIN G. J. Morphologic and histochemical sequences in syphilitic and in tuberculous orchitis in the rabbit. Am J Syph Gonorrhea Vener Dis. 1954 May;38(3):189–202. [PubMed] [Google Scholar]
  11. Wicher V., Wicher K. Studies of rabbit testes infected with Treponema pallidum. II Local synthesis of antibodies. Br J Vener Dis. 1983 Dec;59(6):359–363. doi: 10.1136/sti.59.6.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Zeigler J. A., Jones A. M., Jones R. H., Kubica K. M. Demonstration of extracellular material at the surface of pathogenic T. pallidum cells. Br J Vener Dis. 1976 Feb;52(1):1–8. doi: 10.1136/sti.52.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]

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