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. 1984 Oct;60(5):288–292. doi: 10.1136/sti.60.5.288

Evaluation of an enzyme immunoassay for IgM antibodies to Treponema pallidum in syphilis in man.

F Müller, M Moskophidis
PMCID: PMC1046340  PMID: 6386097

Abstract

An enzyme immunoassay (EIA) for the detection of immunoglobulin M (IgM) antibodies to Treponema pallidum was investigated for specificity and sensitivity. Using the results in serum from 1192 patients with successfully treated syphilis, the assay was calculated to be about 97% specific. As in any other IgM enzyme linked immunosorbent assay (ELISA), rheumatoid factor played an important part in causing false positive results. Pre-absorption of serum with aggregated IgG was therefore necessary to perform the test. Evaluation of the results in serum from 385 patients with untreated primary, secondary, and latent syphilis as well as patients with untreated reinfections showed that the sensitivity of the assay depended on the stage of infection and varied between 98% and 93%. IgM antibody titres were about ten times higher in the EIA than in the indirect immunofluorescence assay using the IgM fractions of serum. From the results it may be concluded that the EIA is an appropriate technique not only for rapid and sensitive measurement of IgM antibodies in most patients with untreated syphilis but also for selecting treponemal IgM non-reactive patients.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Araujo F. G., Remington J. S. IgG antibody suppression of the IgM antibody response to Toxoplasma gondii in newborn rabbits. J Immunol. 1975 Aug;115(2):335–338. [PubMed] [Google Scholar]
  2. Camargo M. E., Ferreira A. W., Mineo J. R., Takiguti C. K., Nakahara O. S. Immunoglobulin G and immunoglobulin M enzyme-linked immunosorbent assays and defined toxoplasmosis serological patterns. Infect Immun. 1978 Jul;21(1):55–58. doi: 10.1128/iai.21.1.55-58.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duermeyer W., van der Veen J. Specific detection of IgM-antibodies by ELISA, applied in hepatitis-A. Lancet. 1978 Sep 23;2(8091):684–685. doi: 10.1016/s0140-6736(78)92802-7. [DOI] [PubMed] [Google Scholar]
  4. Hunter E. F., Farshy C. E., Liska S. L., Cruce D. D., Crawford J. A., Feeley J. C. Sodium desoxycholate-extracted treponemal antigen in an enzyme-linked immunosorbent assay for syphilis. J Clin Microbiol. 1982 Sep;16(3):483–486. doi: 10.1128/jcm.16.3.483-486.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lindenschmidt E. G., Laufs R., Müller F. Microenzyme-linked immunosorbent assay for the detection of specific IgM antibodies in human syphilis. Br J Vener Dis. 1983 Jun;59(3):151–156. doi: 10.1136/sti.59.3.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Moskophidis M., Müller F. Molecular analysis of immunoglobulins M and G immune response to protein antigens of Treponema pallidum in human syphilis. Infect Immun. 1984 Jan;43(1):127–132. doi: 10.1128/iai.43.1.127-132.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Müller F. Der 19S(IgM)-FTA-ABS-Test in der Serodiagnostik der Syphilis. Technik, Fehlermöglichkeiten und diagnostische Aussage. Immun Infekt. 1982 Jan;10(1):23–34. [PubMed] [Google Scholar]
  8. Müller F. Immunological and laboratory aspects of treponematoses. Dermatol Monatsschr. 1984;170(6):357–366. [PubMed] [Google Scholar]
  9. Müller F., Lindenschmidt E. G. Demonstration of specific 19S(IgM) antibodies in untreated and treated syphilis. Comparative studies of the 19S(IgM)-FTA test, the 19S(IgM)-TPHA test, and the solid phase haemadsorption assay. Br J Vener Dis. 1982 Feb;58(1):12–17. doi: 10.1136/sti.58.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Müller F., Oelerich S. Korrelation immunologischer Parameter zu den Stadien der apparenten und er klinisch stummen Syphilis. Dermatol Monatsschr. 1979 Jun;165(6):385–395. [PubMed] [Google Scholar]
  11. Naot Y., Remington J. S. An enzyme-linked immunosorbent assay for detection of IgM antibodies to Toxoplasma gondii: use for diagnosis of acute acquired toxoplasmosis. J Infect Dis. 1980 Nov;142(5):757–766. doi: 10.1093/infdis/142.5.757. [DOI] [PubMed] [Google Scholar]
  12. Roggendorf M., Deinhardt F., Frösner G. G., Scheid R., Bayerl B., Zachoval R. Immunoglobulin M antibodies to hepatitis B core antigen: evaluation of enzyme immunoassay for diagnosis of hepatitis B virus infection. J Clin Microbiol. 1981 Apr;13(4):618–626. doi: 10.1128/jcm.13.4.618-626.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]
  14. Ziegelmaier R., Behrens F., Enders G. Class-specific determination of antibodies against cytomegalo (CMV) and rubella virus by ELISA. J Biol Stand. 1981 Jan;9(1):23–33. doi: 10.1016/s0092-1157(81)80062-5. [DOI] [PubMed] [Google Scholar]

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