Fig 1.
EV production is regulated by growth conditions and growth phase. Schematic representation of the optimized quick protocol used to obtain EVs from cells grown on solid medium (A). EV production from cells grown on different media: SD, YPD, capsule IM, and SAB at 30°C and 37°C, sterol concentration values are expressed per 109 cells for each condition (B). EV production on SD medium at 30°C during the growth curve was analyzed by the amount of total sterol in cellular supernatants when EVs are isolated using the quick protocol, values are expressed per plate of culture during each timepoint (C). Experiments were done with three or more biological replicates. Error bars show means ± SD. Schematic representation created in BioRender.