Fig 6.
Complementation of the hap2Δ and gat5Δ mutant strains restores the phenotypes of EV production and FLC susceptibility. Total sterol contents in EVs produced by WT, mutants, and complemented cells. Sterol concentration values are expressed per 109 cells for each condition (A). Quantification of EV particles using nanoFCM in WT, mutants, and complemented strains (B). FLC susceptibility by disk diffusion assay (25 µg/disk) and RAD80 values obtained by diskImageR (C). FLC susceptibility analysis by spot assay (D). Growth analysis in the presence of cell wall and plasma membrane disturbing agents (CFW, H2O2, and SDS) (E). The experiments were carried out in biological triplicates. Error bars show means ± SD.