Figure 4.

Autolysis in buffer. Strains were grown to log phase and then resuspended in TrisHCl buffer pH 8. Absorbance values at an OD₅₄₀ were taken every 20 minutes for two hours. Both the deletion mutant (ΔmltG) (* p<0.01) and point (mltG*) mutant († p<0.05) had significantly less lysis compared with wildtype (WT). However, the deletion mutant and point mutant were also significantly different at all timepoints except at 20 minutes (p<0.05). There was no difference in lysis between the wildtype and complement strains.