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. 2023 Jul 25;42(36):2701–2709. doi: 10.1038/s41388-023-02782-8

Fig. 3. HUWE1 silencing partially rescues HR capacity in BRCA1-∆11q expressing cells.

Fig. 3

A Confocal microscopy images of IR-induced RAD51 (right) and γH2AX foci (middle panel) from SUM149 cells transfected with HUWE1 siRNA. Cells were transfected with indicated siRNA and irradiated 48 h later. Nuclear foci were detected by immunofluorescence 4 h later and nuclei detected by DAPI staining (left). B Quantification of nuclear foci from experiment described in (A). Foci were counted using CellProfiler. p values were calculated using two-sided t-test. ****p < 0.0001.