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. 2023 Jul 22;26(9):107455. doi: 10.1016/j.isci.2023.107455

Figure 4.

Figure 4

Notch signaling contributed to sympathoexcitation-regulated type H formation

(A) NICD (white), CD31 (green) and EMCN (red) co-immunostaining, counterstained by DAPI (blue), at metaphysis in femurs collected after daily intraperitoneal injections of ISO for one week. Dashed lines indicate the margin of growth plates. Scale bars, 100 μm.

(B) Quantification of NICD+ type H vessel density (%) of (A). N = 3 per group.

(C) NICD (white), CD31 (green) and EMCN (red) co-immunostaining, counterstained by DAPI (blue), at metaphysis in femurs of WT and Adrb2−/− mice. Dashed lines indicate the margin of growth plates. Scale bars, 100 μm.

(D) Quantification of NICD+ type H vessel density (%) of (C). N = 3 per group.

(E) NICD (white), CD31 (green) and EMCN (red) co-immunostaining, counterstained by DAPI (blue), at metaphysis in femurs collected after OVX and daily intraperitoneal injections of PRO for one week. Dashed lines indicate the margin of growth plates. Scale bars, 100 μm.

(F) Quantification of NICD+ type H vessel density (%) of (E). N = 3 per group.

Data represent mean ± SD. Statistical analysis is performed by Student’s t test for two-group comparison or by one-way analysis of variance followed by Newman–Keuls post-hoc tests for multiple comparisons. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.