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. 2023 Apr 6;108(9):2343–2357. doi: 10.3324/haematol.2022.281894

Figure 7.

Figure 7.

Combined inhibition of SIRT3 and cholesterol metabolism increases cell death. (A) Cholesterol levels detected by steady state mass-spectrometry lipidomic analysis in leukemia stem cells (LSC) enriched from 3 primary acute myeloid leukemia (AML) specimens and treated with 10 µM YC8-02 for 4, 8 or 12 hours. Statistical significance was determined using ordinary one-way ANOVA. (B) Cholesteryl-ester levels detected by steady state mass-spectrometry lipidomic analysis in LSC enriched from 3 primary AML specimens and treated with 10 µM YC8-02 for 4, 8 or 12 hours. Quantities are normalized to baseline control. Statistical significance was determined using ordinary one-way ANOVA. (C) Viability of LSC and AML blasts enriched from 3 primary AML specimens and treated with 10 µM YC8-02 alone or in combination with 0.5 µM dipyridamole. Statistical significance was determined using two-way ANOVA. All error bars represent standard deviation. *P<0.05, **P<0.01, ***P<0.005, ****P<0.001; ns: not significant.