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. 1980 Jun;17(3):187–190. doi: 10.1136/jmg.17.3.187

Alkaline phosphatase activity of normal and cystic fibrosis fibroblasts.

D A Aitken, A Hoogeveen
PMCID: PMC1048544  PMID: 7401129

Abstract

Alkaline phosphatase (ALP) activities were compared in fibroblasts from three cystic fibrosis patients and two normal controls after culturing the cells in normal growth medium and in medium containing Tamm-Horsfall glycoprotein, isoproterenol, and theophylline. No consistent alterations in ALP activities were noted, either between the same cell lines grown under different conditions, or between normal and cystic cell lines. It is concluded that it is not possible to use changes in ALP activity in cultured cells for the prenatal diagnosis of cystic fibrosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brojer B., Moss D. W. Changes in the alkaline phosphatase activity of serum samples after thawing and after reconstitution from the lyophilized state. Clin Chim Acta. 1971 Dec;35(2):511–513. doi: 10.1016/0009-8981(71)90232-4. [DOI] [PubMed] [Google Scholar]
  2. Buchwald M. Abnormal levels of 3':5'-cyclic AMP in isoproterenol-stimulated fibroblasts from patients with cystic fibrosis. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2899–2903. doi: 10.1073/pnas.73.8.2899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. COX R. P., PONTECORVO G. Induction of alkaline phosphatase by substrates in established cultures of cells from individual human donors. Proc Natl Acad Sci U S A. 1961 Jun 15;47:839–845. doi: 10.1073/pnas.47.6.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis P. B., Braunstein M., Jay C. Decreased adenosine 3':5'-monophosphate response to isoproterenol in cystic fibrosis leukocytes. Pediatr Res. 1978 Jun;12(6):703–707. doi: 10.1203/00006450-197806000-00005. [DOI] [PubMed] [Google Scholar]
  5. Galjaard H., Hoogeveen A., Keijzer W., De Wit-Verbeek E., Vlek-Noot C. The use of quantiatative cytochemical analyses in rapid prenatal detection and somatic cell genetic studies of metabolic diseases. Histochem J. 1974 Sep;6(5):491–509. doi: 10.1007/BF01003266. [DOI] [PubMed] [Google Scholar]
  6. Hösli P., Erickson R. P., Vogt E. Prospects for prenatal diagnosis of cystic fibrosis: induction of biochemical abnormalities in fibroblasts from patients with cystic fibrosis by a urinary glycoprotein. Biochem Biophys Res Commun. 1976 Nov 22;73(2):209–216. doi: 10.1016/0006-291x(76)90695-1. [DOI] [PubMed] [Google Scholar]
  7. Hösli P. Quantitative assays of enzyme activity in single cells: early prenatal diagnosis of genetic disorders. Clin Chem. 1977 Aug;23(8):1476–1484. [PubMed] [Google Scholar]
  8. Hösli P., Vogt E. Cystic fibrosis: leakage of lysosomal enzymes and of alkaline phosphatase into the extracellular space. Biochem Biophys Res Commun. 1977 Dec 7;79(3):741–748. doi: 10.1016/0006-291x(77)91174-3. [DOI] [PubMed] [Google Scholar]
  9. Hösli P., Vogt E. Reliable detection of cystic fibrosis in skin-derived fibroblast cultures. Hum Genet. 1978 Mar 17;41(2):169–173. doi: 10.1007/BF00273098. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Lundgren E. Conditions for induction of alkaline phosphatase in cultured human fetal skin fibroblasts. Exp Cell Res. 1977 Nov;110(1):25–30. doi: 10.1016/0014-4827(77)90265-8. [DOI] [PubMed] [Google Scholar]
  12. Mulivor R. A., Mennuti M., Zackai E. H., Harris H. Prenatal diagnosis of hypophosphatasia; genetic, biochemical, and clinical studies. Am J Hum Genet. 1978 May;30(3):271–282. [PMC free article] [PubMed] [Google Scholar]
  13. TAMM I., HORSFALL F. L., Jr A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses. J Exp Med. 1952 Jan;95(1):71–97. doi: 10.1084/jem.95.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Vanneuville F. J., Van Elsen A. F., Leroy J. G. Alkaline phosphatase: presence and behaviour in human diploid fibroblasts. Arch Int Physiol Biochim. 1975 Dec;83(5):1017–1018. [PubMed] [Google Scholar]
  15. Vanneuville F. J., Van Elsen A. F., Leroy J. G. Alkaline phosphatase: properties in crude homogenates of human diploid fibroblasts [proceedings]. Biochem Soc Trans. 1977;5(4):1117–1117. doi: 10.1042/bst0051117. [DOI] [PubMed] [Google Scholar]

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