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. 1983 Oct;20(5):372–376. doi: 10.1136/jmg.20.5.372

Spontaneous and mutagen induced sister chromatid exchange in multiple sclerosis.

Vijayalaxmi, M S Newton, C M Steel, H J Evans, B Pentland
PMCID: PMC1049153  PMID: 6417337

Abstract

Spontaneous and mutagen induced sister chromatid exchange (SCE) frequencies have been studied in nine patients with multiple sclerosis and in nine age and sex matched healthy controls. The incidence of spontaneous SCE in lymphocytes of the MS patients was significantly greater, by about 50%, than in those of the control subjects. When exposed to mitomycin C (MMC) or ethyl methane sulfonate (EMS) in vitro, cells from both groups showed typical dose dependent increases in SCE frequency, with yields from MS patients slightly higher than from controls. The higher SCE yields in mutagen treated MS cells relative to controls is considered to reflect initial basal differences between the cell types, so that MS cells are not intrinsically hypersensitive to mutagen treatment.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acheson E. D. Epidemiology of multiple sclerosis. Br Med Bull. 1977 Jan;33(1):9–14. doi: 10.1093/oxfordjournals.bmb.a071407. [DOI] [PubMed] [Google Scholar]
  2. Andrews A. D., Barrett S. F., Robbins J. H. Relation of D.N.A. repair processes to pathological ageing of the nervous system in xeroderma pigmentosum. Lancet. 1976 Jun 19;1(7973):1318–1320. doi: 10.1016/s0140-6736(76)92652-0. [DOI] [PubMed] [Google Scholar]
  3. Bach M. A., Phan-Dinh-Tuy F., Tournier E., Chatenoud L., Bach J. F., Martin C., Degos J. D. Deficit of suppressor T cells in active multiple sclerosis. Lancet. 1980 Dec 6;2(8206):1221–1223. doi: 10.1016/s0140-6736(80)92480-0. [DOI] [PubMed] [Google Scholar]
  4. Bloom B. R. Immunological changes in multiple sclerosis. Nature. 1980 Sep 25;287(5780):275–276. doi: 10.1038/287275a0. [DOI] [PubMed] [Google Scholar]
  5. Cleaver J. E., Bootsma D. Xeroderma pigmentosum: biochemical and genetic characteristics. Annu Rev Genet. 1975;9:19–38. doi: 10.1146/annurev.ge.09.120175.000315. [DOI] [PubMed] [Google Scholar]
  6. Cremer N. E., Johnson K. P., Fein G., Likosky W. H. Comprehensive viral immunology of multiple sclerosis. II. Analysis of serum and CSF antibodies by standard serologic methods. Arch Neurol. 1980 Oct;37(10):610–615. doi: 10.1001/archneur.1980.00500590034003. [DOI] [PubMed] [Google Scholar]
  7. Dau P. C., Peterson R. D. Transformation of lymphocytes from patients with multiple sclerosis. Use of encephalitogen of human origin, with a report of a trial of immunosuppressive therapy in multiple sclerosis. Arch Neurol. 1970 Jul;23(1):32–40. doi: 10.1001/archneur.1970.00480250036006. [DOI] [PubMed] [Google Scholar]
  8. Dausset J., Hors J. Some contributions of the HL-A complex to the genetics of human diseases. Transplant Rev. 1975;22:44–74. doi: 10.1111/j.1600-065x.1975.tb01551.x. [DOI] [PubMed] [Google Scholar]
  9. Field E. J., Meyer-Rienecker H. J., Shenton B. K., Jenssen H. L., Köhler H. Anomalous lymphocyte-antigen reaction in relatives of multiple sclerosis patients. A study of a possible genetic factor in the disease. J Neurol. 1977 Sep 12;216(2):135–146. doi: 10.1007/BF00312947. [DOI] [PubMed] [Google Scholar]
  10. Fraser K. B. Multiple sclerosis: a virus disease? Br Med Bull. 1977 Jan;33(1):34–39. doi: 10.1093/oxfordjournals.bmb.a071392. [DOI] [PubMed] [Google Scholar]
  11. Gipps E., Kidson C. Ionising radiation sensitivity in multiple sclerosis. Lancet. 1981 Apr 25;1(8226):947–947. doi: 10.1016/s0140-6736(81)91644-5. [DOI] [PubMed] [Google Scholar]
  12. Haire M., Fraser K. B., Millar J. H. Measles and other virus-specific immunoglobulins in multiple sclerosis. Br Med J. 1973 Sep 22;3(5881):612–615. doi: 10.1136/bmj.3.5881.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jensen M. K. Lymphocyte transformation in multiple sclerosis. Acta Neurol Scand. 1968;44(2):200–206. doi: 10.1111/j.1600-0404.1968.tb05566.x. [DOI] [PubMed] [Google Scholar]
  14. Jersild C., Svejgaard A., Fog T., Ammitzboll T. HL-A antigens and diseases. I. Multiple sclerosis. Tissue Antigens. 1973;3(4):243–250. doi: 10.1111/j.1399-0039.1973.tb01002.x. [DOI] [PubMed] [Google Scholar]
  15. Kaplan M. E., Clark C. An improved rosetting assay for detection of human T lymphocytes. J Immunol Methods. 1974 Jul;5(2):131–135. doi: 10.1016/0022-1759(74)90003-9. [DOI] [PubMed] [Google Scholar]
  16. Knight S. C., Harding B., Burman S., Mertin J. Cell number requirements for lymphocyte stimulation in vitro: changes during the course of multiple sclerosis and the effects of immunosuppression. Clin Exp Immunol. 1981 Oct;46(1):61–69. [PMC free article] [PubMed] [Google Scholar]
  17. Knight S. C., Lance E. M., Abbosh J., Munro A., O'Brien J. Intensive immunosuppression in patients with disseminated sclerosis. III. Lymphocyte response in vitro. Clin Exp Immunol. 1975 Jul;21(1):23–31. [PMC free article] [PubMed] [Google Scholar]
  18. Lambert B., Ehrnst A., Hansson K., Lindblad A., Morad M., Werelius B. Sister chromatid exchange in peripheral lymphocytes of subjects vaccinated against measles. Hum Genet. 1979 Sep;50(3):291–296. doi: 10.1007/BF00399395. [DOI] [PubMed] [Google Scholar]
  19. Lamoureux G., Giard N., Jolicoeur R., Toughlian V., Desrosiers M. Immunological features in multiple sclerosis. Br Med J. 1976 Jan 24;1(6003):183–186. doi: 10.1136/bmj.1.6003.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lindblad A., Lambert B. Relation between sister chromatid exchange, cell proliferation and proportion of B and T cells in human lymphocyte cultures. Hum Genet. 1981;57(1):31–34. doi: 10.1007/BF00271163. [DOI] [PubMed] [Google Scholar]
  21. Lisak R. P., Levinson A. I., Zweiman B., Abdou N. I. T and B lymphocytes in multiple sclerosis. Clin Exp Immunol. 1975 Oct;22(1):30–34. [PMC free article] [PubMed] [Google Scholar]
  22. Martin J. R. Herpes simplex virus types 1 and 2 and multiple sclerosis. Lancet. 1981 Oct 10;2(8250):777–781. doi: 10.1016/s0140-6736(81)90188-4. [DOI] [PubMed] [Google Scholar]
  23. McDonald W. I., Halliday A. M. Diagnosis and classification of multiple sclerosis. Br Med Bull. 1977 Jan;33(1):4–9. [PubMed] [Google Scholar]
  24. Mertin J., Meade C. J. Relevance of fatty acids in multiple sclerosis. Br Med Bull. 1977 Jan;33(1):67–71. doi: 10.1093/oxfordjournals.bmb.a071399. [DOI] [PubMed] [Google Scholar]
  25. Ockey C. H. Differences between "spontaneous" and induced sister-chromatid exchanges with fixation time and their chromosome localization. Cytogenet Cell Genet. 1980;26(2-4):223–235. doi: 10.1159/000131443. [DOI] [PubMed] [Google Scholar]
  26. Oger J. F., Arnason B. G., Wray S. H., Kistler J. P. A study of B and T cells in multiple sclerosis. Neurology. 1975 May;25(5):444–447. doi: 10.1212/wnl.25.5.444. [DOI] [PubMed] [Google Scholar]
  27. Paterson M. C., Smith P. J. Ataxia telangiectasia: an inherited human disorder involving hypersensitivity to ionizing radiation and related DNA-damaging chemicals. Annu Rev Genet. 1979;13:291–318. doi: 10.1146/annurev.ge.13.120179.001451. [DOI] [PubMed] [Google Scholar]
  28. Perry P., Wolff S. New Giemsa method for the differential staining of sister chromatids. Nature. 1974 Sep 13;251(5471):156–158. doi: 10.1038/251156a0. [DOI] [PubMed] [Google Scholar]
  29. Poskanzer D. C., Prenney L. B., Sheridan J. L., Kondy J. Y. Multiple sclerosis in the Orkney and Shetland Islands. I: Epidemiology, clinical factors, and methodology. J Epidemiol Community Health. 1980 Dec;34(4):229–239. doi: 10.1136/jech.34.4.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Reinherz E. L., Weiner H. L., Hauser S. L., Cohen J. A., Distaso J. A., Schlossman S. F. Loss of suppressor T cells in active multiple sclerosis. Analysis with monoclonal antibodies. N Engl J Med. 1980 Jul 17;303(3):125–129. doi: 10.1056/NEJM198007173030303. [DOI] [PubMed] [Google Scholar]
  31. Robbins J. H. Significance of repair of human DNA: evidence from studies of xeroderma pigmentosum. J Natl Cancer Inst. 1978 Sep;61(3):645–656. [PubMed] [Google Scholar]
  32. Sandberg-Wollheim M., Turesson I. Lymphocyte subpopulations in the cerebrospinal fluid and peripheral blood in patients with multiple sclerosis. Scand J Immunol. 1975;4(8):831–836. doi: 10.1111/j.1365-3083.1975.tb03724.x. [DOI] [PubMed] [Google Scholar]
  33. Santesson B., Lindahl-Kiessling K., Mattsson A. SCE in B and T lymphocytes. Possible implications for Bloom's syndrome. Clin Genet. 1979 Aug;16(2):133–135. doi: 10.1111/j.1399-0004.1979.tb00862.x. [DOI] [PubMed] [Google Scholar]
  34. Sutherland G. R., Baker E., Seshadri R. S., Black A. Increased sister-chromatid exchange in multiple sclerosis. N Engl J Med. 1980 Nov 6;303(19):1126–1126. doi: 10.1056/NEJM198011063031922. [DOI] [PubMed] [Google Scholar]
  35. Taylor A. M., Harnden D. G., Arlett C. F., Harcourt S. A., Lehmann A. R., Stevens S., Bridges B. A. Ataxia telangiectasia: a human mutation with abnormal radiation sensitivity. Nature. 1975 Dec 4;258(5534):427–429. doi: 10.1038/258427a0. [DOI] [PubMed] [Google Scholar]
  36. Taylor A. M. Unrepaired DNA strand breaks in irradiated ataxia telangiectasia lymphocytes suggested from cytogenetic observations. Mutat Res. 1978 Jun;50(3):407–418. doi: 10.1016/0027-5107(78)90045-3. [DOI] [PubMed] [Google Scholar]
  37. Trotter J. L., Rodey G. E., Gebel H. M. Azathioprine decreases suppressor T cells in patients with multiple sclerosis. N Engl J Med. 1982 Feb 11;306(6):365–366. doi: 10.1056/NEJM198202113060615. [DOI] [PubMed] [Google Scholar]
  38. Van den Noort S., Stjernholm R. L. Lymphotoxic activity in multiple sclerosis serum. Neurology. 1971 Aug;21(8):783–793. doi: 10.1212/wnl.21.8.783. [DOI] [PubMed] [Google Scholar]
  39. ter Meulen V., Koprowski H., Iwasaki Y., Käckell Y. M., Müller D. Fusion of cultured multiple-sclerosis brain cells with indicator cells: presence of nucleocapsids and virions and isolation of parainfluenza-type virus. Lancet. 1972 Jul 1;2(7766):1–5. doi: 10.1016/s0140-6736(72)91273-1. [DOI] [PubMed] [Google Scholar]

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