Jia Zhang
Jia Zhang
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
1,2,3,4,†,
Xiaolu Su
Xiaolu Su
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
1,2,3,4,†,
Yefei Wang
Yefei Wang
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
5
CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2,3,4,5,†,
Xiaohang Wang
Xiaohang Wang
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
1,2,3,4,
Shiqi Zhou
Shiqi Zhou
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
1,2,3,4,
Hui Jia
Hui Jia
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
1,2,3,4,
Xiaoyan Jing
Xiaoyan Jing
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
1,2,3,4,
Yanhai Gong
Yanhai Gong
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
1,2,3,4,
Jichao Wang
Jichao Wang
6
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, China
6,*,
Jian Xu
Jian Xu
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
1,2,3,4,*
1
Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
2
Shandong Energy Institute, Qingdao, Shandong, China
3
Qingdao New Energy Shandong Laboratory, Qingdao, Shandong, China
4
University of Chinese Academy of Sciences, Beijing, China
5
CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China
6
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, China
✉*Correspondence: Jichao Wang, wangjichao@ustc.edu; Jian Xu, xujian@qibebt.ac.cn
†
These authors have contributed equally to this work and share first authorship
Received 2023 Jul 20; Accepted 2023 Aug 8; Collection date 2023.
Keywords: single-cell genome amplification, phi29 DNA polymerase, disulfide bond, GB1 fusion protein, process engineering high-efficiency phi29 DNA polymerase
Copyright © 2023 Zhang, Su, Wang, Wang, Zhou, Jia, Jing, Gong, Wang and Xu.
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