Skip to main content
NCBI home page
Journal of Medical Genetics logoLink to Journal of Medical Genetics
. 1986 Jun;23(3):210–216. doi: 10.1136/jmg.23.3.210

DNA polymorphic haplotypes on the short arm of chromosome 11 and the inheritance of type I diabetes mellitus.

G A Ferns, G A Hitman, R Trembath, L Williams, A Tarn, E A Gale, D J Galton
PMCID: PMC1049629  PMID: 3014147

Abstract

The linked polymorphic loci 5' to the insulin gene and 3' to the c-Harvey-ras-1 (c-Ha-ras) gene, both localised to the short arm of chromosome 11, have been studied in 14 type I diabetic pedigrees. The use of a cloned gene probe corresponding to the polymorphic locus adjacent to the insulin gene, in combination with the restriction endonuclease PvuII, has permitted an improvement in the resolution of sizes of insert at this locus. An MspI restriction fragment length polymorphism at the c-Ha-ras proto-oncogene locus (4 cM upstream from the insulin gene) was used to identify parental insulin gene related alleles unambiguously, and subsequently a pedigree analysis was performed to determine whether subclasses of inserts at this locus track with insulin dependent diabetes. Segregation analysis demonstrated no linkage between the polymorphic loci 5' to the insulin gene, nor 3' to the c-Ha-ras, and type I diabetes. However, a similar analysis confirmed an association between the HLA locus chromosome 6 and insulin dependent diabetes.

Full text

PDF
210

Images in this article

211Image
on p.211
211Image
on p.211

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell G. I., Horita S., Karam J. H. A polymorphic locus near the human insulin gene is associated with insulin-dependent diabetes mellitus. Diabetes. 1984 Feb;33(2):176–183. doi: 10.2337/diab.33.2.176. [DOI] [PubMed] [Google Scholar]
  2. Bell G. I., Selby M. J., Rutter W. J. The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequences. Nature. 1982 Jan 7;295(5844):31–35. doi: 10.1038/295031a0. [DOI] [PubMed] [Google Scholar]
  3. Fearon E. R., Antonarakis S. E., Meyers D. A., Levine M. A. c-Ha-ras-1 oncogene lies between beta-globin and insulin loci on human chromosome 11p. Am J Hum Genet. 1984 Mar;36(2):329–337. [PMC free article] [PubMed] [Google Scholar]
  4. Hitman G. A., Tarn A. C., Winter R. M., Drummond V., Williams L. G., Jowett N. I., Bottazzo G. F., Galton D. J. Type 1 (insulin-dependent) diabetes and a highly variable locus close to the insulin gene on chromosome 11. Diabetologia. 1985 Apr;28(4):218–222. doi: 10.1007/BF00282236. [DOI] [PubMed] [Google Scholar]
  5. Jeffreys A. J., Wilson V., Thein S. L. Individual-specific 'fingerprints' of human DNA. Nature. 1985 Jul 4;316(6023):76–79. doi: 10.1038/316076a0. [DOI] [PubMed] [Google Scholar]
  6. Kunkel L. M., Smith K. D., Boyer S. H., Borgaonkar D. S., Wachtel S. S., Miller O. J., Breg W. R., Jones H. W., Jr, Rary J. M. Analysis of human Y-chromosome-specific reiterated DNA in chromosome variants. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1245–1249. doi: 10.1073/pnas.74.3.1245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Thomson G. A two locus model for juvenile diabetes. Ann Hum Genet. 1980 May;43(4):383–398. doi: 10.1111/j.1469-1809.1980.tb01572.x. [DOI] [PubMed] [Google Scholar]
  8. Walker M. D., Edlund T., Boulet A. M., Rutter W. J. Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes. Nature. 1983 Dec 8;306(5943):557–561. doi: 10.1038/306557a0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Medical Genetics are provided here courtesy of BMJ Publishing Group

RESOURCES