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. 2023 Aug 31;26(10):107803. doi: 10.1016/j.isci.2023.107803

Figure 4.

Figure 4

In vitro reconstitution of the sulfo-ASDO pathway showing the formation of glucose

(A) Extracted ion chromatograms (m/z (−) 179.0, the predicted mass of glucose monoanion), showing product peaks in the full assay coeluting with glucose standard.

(B) ESI (−) m/z spectrum of the product peak showing a species with m/z (−) 179.0 corresponding to glucose.

(C) Enzyme activity assay monitoring NADPH consumption accompanying 6-dehydroglucose reduction by MaSquF. See also Figure S1.