In vitro reconstitution of the sulfo-ASDO pathway showing the formation of glucose
(A) Extracted ion chromatograms (m/z (−) 179.0, the predicted mass of glucose monoanion), showing product peaks in the full assay coeluting with glucose standard.
(B) ESI (−) m/z spectrum of the product peak showing a species with m/z (−) 179.0 corresponding to glucose.
(C) Enzyme activity assay monitoring NADPH consumption accompanying 6-dehydroglucose reduction by MaSquF. See also Figure S1.