Figure 5.

Growth of M. ushuaiensis in minimal medium containing different carbon sources

(A) Tubes 1, 2, and 3 show cells grown in media with SQ, glucose or no additional carbon source. Detect the optical density at 600 nm. Data are represented as mean ± SD.

(B) Quantification of sulfite formed in cultures 1, 2, and 3 by a colorimetric fuchsin assay. Inset: Photographs of the respective assays.

(C) Optical density of M. ushuaiensis culture (blue) and concentration of SQ ([SQ]) (black) and change in concentration of sulfite (Δ[sulfite]) (red), with respect to time.

(D) qPCR analyses of the transcription levels of SqoD and SquF. The transcriptional levels of genes of interest were normalized by that of the 16S rRNA. The induction by SQ was displayed in comparison with the transcriptional data from glucose-grown cells. The error bars represent the standard deviation of three individual experiments. Data are represented as mean ± SD. Significance was assessed by an ordinary one-way ANOVA test (∗∗∗∗p value <0.0001). See also Figures S3 and S8 and Table S2.