Figure 3.
Chemogenetic regulation of vlPAGGABA neurons modulates arousal during sevoflurane anesthesia
(A) Injection of chemogenetic viruses into vlPAG and EEG recording configuration.
(B) The images show GABAergic neurons (green) expressing chemogenetic virus (mCherry, red).
(C) Activation of vlPAGGABA significantly shortened RORR time but did not change LORR.
(D) The representative EEG spectrum of the control group (hM3Dq-group) and the experimental group (hM3Dq+ group) in 2.0% (top) and 1.5% (down) sevoflurane anesthesia.
(E) Under 2.0% sevoflurane anesthesia, the hM3Dq+ group exhibited a significantly lower BSR of EEG compared to the hM3Dq-group.
(F) Power spectral analysis of EEG signals shows that the percentage of power in the δ band decreased significantly. In contrast, that in the γ band increased in the hM3Dq+ group more than in the hM3Dq-group during 1.5% sevoflurane anesthesia.
(G) Inhibition of vlPAGGABA significantly prolonged RORR time but had no significant effect on LORR time.
(H) The representative EEG spectrum of the control group (hM4Di-group) and the experimental group (hM4Di+ group) in 2.0% (top) and 1.5% (down) sevoflurane anesthesia.
(I) Under 2.0% sevoflurane anesthesia, the hM4Di+ group demonstrated a significantly higher BSR of EEG compared to the hM4Di-group.
(J) Under 1.5% sevoflurane anesthesia, the hM4Di+ group displayed a significantly higher BSR of EEG than the hM4Di-group. The data is presented as the mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.