Figure 6.

GABA fluorescent sensor in VTA can follow transients in intact neural circuits

(A) Schematic diagram of virus injection.

(B) Virus expression indicates pyramidal neuron projections from the mPFC to the vlPAG and GABA fluorescent sensor expression in VTA.

(C)Schematic of the EEG recording configuration and sensor recording.

(D) Schematic diagram of light stimulation and signal recording mode.

(E) One second of light stimulation was accompanied by a second increase in the sensor fluorescence signal.

(F) After the beginning of 2.0% sevoflurane anesthesia, the activity of GABA sensor signals gradually decreased (top), and the heatmaps changes for five mice (bottom).

(G) The ΔF/F value of GABA sensor signals decreased significantly after the beginning of anesthesia.

(H) After 2.0% sevoflurane anesthesia, the activity of GABA sensor signals gradually increased (top), and the heatmap changes of five mice (bottom).

(I) The ΔF/F value of GABA sensor signals increased markedly after anesthesia.

(J) Representative elevation changes of GABA sensor signal in VTA and corresponding EEG spectrum during anesthesia.

(K) Trend diagram of the percentage of power in the different frequency bands, related to (J). Data are shown as the mean ± SEM, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.