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. Author manuscript; available in PMC: 2024 Aug 3.
Published in final edited form as: Cell. 2023 Jul 7;186(16):3350–3367.e19. doi: 10.1016/j.cell.2023.06.004

Figure 2. F0502B selectively binds to α-Syn aggregates in vitro and ex vivo.

Figure 2.

(A) The binding of ThT, EU05-02B, and F0502B to α-Syn, Aβ, and Tau PFFs. ThT binding with the same PFF concentration showed a binding peak at 485 nm. EU05-02B and F0502B showed stronger binding affinity with α-Syn PFFs than with Aβ and Tau PFFs.

(B and C) Characterization of EU05-02B selectivity for aggregates in cultured neurons, mouse SN region expressing α-Syn A53T, and brain slices from DLB (cortex) and MSA (SN) patients (arrows show co-localization signals). Scale bars, 20 μm.

(D) The levels of F0502B in the plasma and brain homogenates of male ICR mice that received i.v. injection of 5 mg/kg F0502B. The B/P ratio was determined at different time points. Error bars represent the mean ± SD. Data are representative of 3 mice at every time point.

(E) Staining of inclusions in WT, 5xFAD, Tau P301S, 3xTg (cortex), and AAV-α-Syn A53T-injected mice (SN) intravenously administered F0502B (2 mg/kg). The tissues were prepared at 2 h after tracer administration. Scale bars, 20 μm.

See also Figures S3 and S4.