Table 1.
Data collection and refinement statistics.
| Data collection | |
|---|---|
| Microscope | Titan Krios |
| Acceleration Voltage (kV) | 300 |
| Detector | K3 |
| Software | SerialEM 3.8 |
| Magnification | 105,000x |
| Pixel size at detector (Å/px) | 0.83 |
| Defocus range (μm) | −1.20 to −2.40 |
| Total dose (e−/ Å2) | 52 |
| Exposure time (sec) | 4.50 |
| Number of movie frames | 50 |
| Reconstruction | |
| Usable micrograph | 5901 |
| Box size (pixel) | 180 |
| Total extracted segments | 1,696,844 |
| Number of segments after 2D | 125,446 |
| Number of segments after 3D | 45,674 |
| Symmetry imposed | C1 |
| Helical rise (Å) | 4.75 |
| Helical twist (°) | −1.00 |
| Crossover length (Å) | t 855 |
| Map sharpening B-factor (Å2) | −143.25 |
| Map resolution (Å; FSC=0.143) | 3.88 |
| Model composition | |
| Non-hydrogen atoms | 4,096 |
| Protein residues | 504 |
| Number of chains | 4.00 |
| Water | 0.00 |
| Ligands (Acetylated) | 8.00 |
| Model validation | |
| Map CC (mask) | 0.74 |
| MolProbity score | 2.14 |
| Clash score | 25.23 |
| R.M.S deviations bonds (Å) | 0.007 |
| R.M.S deviations angle (°) | 1.291 |
| Rotamer outliers (%) | 0.00 |
| Ramachandran plot (favored/allowed/outliers) | (96.30/3.70/0.00) |
| Cβ outliers | 0.00 |
| CaBLAM outliers (%) | 2.56% |