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. Author manuscript; available in PMC: 2024 Jul 24.
Published in final edited form as: Curr Biol. 2023 Jul 14;33(14):3056–3064.e5. doi: 10.1016/j.cub.2023.06.051

Figure 3. SPE-36 is a secreted protein and acts cell autonomously.

Figure 3.

GFP-tagged proteins that are secreted from muscle cells can be taken up by the coelomocytes. GFP attached to a signal sequence (ssGFP)30, SPE-36 tagged with GFP, and SPE-9 tagged with GFP were each expressed in body wall muscle cells using the myo-3 promoter to drive expression. When expressed under the myo-3 promoter, SPE-36::GFP is present in coelomocytes (arrowheads) while the transmembrane protein SPE-9::GFP is not observed in these cells. (A-B) ssGFP (C-D) SPE-36::GFP (E-F) SPE-9::GFP. Scale bars = 10 μm (G) Hermaphrodites and males were crossed for 24 hours at 20°C. After 24 hours adults were removed from the plates. The number of progeny on each plate was counted 3 days later. For crosses with dpy-5 hermaphrodites the Dpy phenotype was used to distinguish between self-progeny (Dpy) and outcross progeny (non-Dpy). The average number of progeny for each cross is reported. n = number of crosses

See also Figure S2.