KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| InVivoMAb anti-mouse PD-1 (CD279, clone: RMP1-14) | Bio X Cell | Cat# BE0146; RRID:AB_10949053 |
| InVivoMAb rat IgG2a isotype control | Bio X Cell | Cat# BE0089; RRID:AB_1107769 |
| InVivoMAb anti-mouse PD-L1 (B7-H1) | Bio X Cell | Cat# BE0101; RRID:AB_10949073 |
| InVivoMAb rat IgG2b isotype control | Bio X Cell | Cat# BE0090; RRID:AB_1107780 |
| Rabbit anti-PD-1 | Sigma | Cat# PRS4065; RRID:AB_1855098 |
| Mouse anti-NeuN | Millipore | Cat# MAB377; RRID:AB_2298772 |
| Guinea pig anti-NeuN | Sigma | Cat# ABN90P; RRID:AB_2341095 |
| Goat anti-tdTomato | Origene | Cat# AB8181-200; RRID:AB_2722750 |
| Rabbit anti-Iba1 | Wako | Cat# 019-19741; RRID:AB_839504 |
| Mouse anti-GFAP | Millipore | Cat# MAB360; RRID:AB_11212597 |
| Anti-mouse CD16/32 | Biolegend | Cat# 156603; RRID:AB_2783137 |
| CD45 Monoclonal Antibody (30-F11), FITC | eBioscience | Cat# 11-0451-82; RRID:AB_465050 |
| PE anti-mouse/human CD 11b Antibody | Biolegend | Cat# 101207; RRID:AB_312790 |
| Monoclonal Anti-Vglut1 - APC antibody | Sigma | Cat# SAB5200268-100UG |
| APC/Cyanine7 anti-mouse CD279 (PD-1) Antibody | BioLegend | Cat# 135223; RRID:AB_2563522 |
| APC/Cy7 anti-mouse/human CD11b | Biolegend | Cat# 101225; RRID:AB_830641 |
| PE anti-mouse CD279 (PD-1) | Biolegend | Cat# 135205; RRID:AB_1877232 |
| CaMKII alpha | Thermo Fisher Scientific | Cat# PA5-19128; RRID:AB_10986857 |
| Nivolumab (human PD-1) | Bristol-Myers Squibb | Cat# NDC 0003-3772-11 |
| Rabbit anti-PD-L1 | Abcam | Cat# ab233482; RRID:AB_2811045 |
| Anti-mouse IgG (H+L), Biotinylated Antibody | Cell Signaling Technology | Cat# 14709; RRID:AB_2798582 |
| Anti-Human vivo 680 | Sigma | Cat# I2511; RRID:AB_1163604 |
| FITC Rat IgG2a, κ Isotype Control | BD Phanningen | Cat# 557228; RRID:AB_396607 |
| Isotype Control Antibody, rat IgG1, PE | Miltenyl Biotech | Cat# 130-123-746; RRID:AB_2857627 |
| Bacterial and virus strains | ||
| AAV-CamKII-mPDCD1-IRES-eGFP-WPRE | Vector Biolabs | N/A |
| AAV-CamKII-mCherry-Cre | UNC Vector Core | N/A |
| AAV-CamKII-eGFP-WPRE | James M. Wilson lab (University of Pennsylvania) | Cat# 105541-AAV9; RRID:Addgene_105541 |
| Biological samples | ||
| Human hippocampus tissue | National Disease Research interchange (NDRI) See Table S2 for sex and age information | N/A |
| Human plasma and CSF sample | MAD-PIA clinical trial See Table S2 for sex and age information | N/A |
| Monkey hippocampus tissue | Wake Forest University See Table S3 for sex and age information | N/A |
| Monkey serum and CSF sample | Wake Forest University See Table S3 for sex and age information | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Nissl | Thermo Scientific | Cat# N21483 |
| Neurobiotin | Vector Laboratories | Cat# SP-1120 |
| Alexa Fluor® 594 streptavidin | Invitrogen | Cat# S32356 |
| Tetrodotoxin (TTX) | Tocris | Cat# 1078 |
| Picrotoxin | Tocris | Cat# 1128 |
| CNQX | Tocris | Cat# 0190 |
| DL-AP5 | Tocris | Cat# 0105 |
| U0126 | InvivoGen | Cat# tlrl-u0126 |
| Human IgG4 | Abcam | Cat# ab90286 |
| Human PD-1-Biotin | Acrobiosystems | Cat# EP101 |
| Human PD-L1-Biotin | Acrobiosystems | Cat# EP158 |
| Recombinant mouse PD-L1 protein | Abcam | Cat# ab221310 |
| Recombinant Mouse IgG2a protein | Abcam | Cat# ab300223 |
| Recombinant Rhesus monkey PD-L1 protein | Abcam | Cat# ab201421 |
| RNAscope probe mouse Pdcd1 | Advanced Cell Diagnostics | Cat# 416781 |
| RNAscope probe human PDCD1 | Advanced Cell Diagnostics | Cat# 602021 |
| RNAscope probe Mmu PDCD1 | Advanced Cell Diagnostics | N/A |
| RNAscope probe mouse Cd274 | Advanced Cell Diagnostics | Cat# 420501 |
| RNAscope Negative control probe | Advanced Cell Diagnostics | Cat# 700141 |
| Critical commercial assays | ||
| RNAscope® Multiplex Fluorescent Reagent Kit v2 | Advanced Cell Diagnostics | Cat# 323100 |
| TSA™ Cyanine 3&5, TMR, Fluorescein Evaluation Kit | PerkinElmer | Cat# NEL760001KT |
| Zombie Violet™ Fixable Viability Kit | Biolegend | Cat# 423113 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J (Wildtype) | The Jackson Laboratory | Stock No: 000664; RRID:IMSR_JAX:000664 |
| Mouse: Pdcd1−/− | The Jackson Laboratory | Stock No: 021157; RRID:IMSR_JAX:021157 |
| Mouse: Camk2a-Cre | The Jackson Laboratory | Stock No: 005359; RRID:IMSR_JAX:005359 |
| Mouse: Vgat-Cre | The Jackson Laboratory | Stock No: 028862; RRID:IMSR_JAX:028862 |
| Mouse: Cx3cr1CreER | The Jackson Laboratory | Stock No: 020940; RRID:IMSR_JAX:020940 |
| Mouse: Pdcd1fl/fl | Richard Palmiter lab (University of Washington) Generated only for this study | N/A |
| Mouse: Pdcd1-Cre | Cyagen Biosciences Inc. | N/A |
| Mouse: Ai9 | The Jackson Laboratory | Stock No: 007909; RRID:IMSR_JAX:007909 |
| Oligonucleotides | ||
| See Table S4 for Sequences of genotyping primers. | N/A | N/A |
| Software and algorithms | ||
| Image J | NIH | https://imagej.nih.gov/ij/ |
| Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Zen lite | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| Leica LAS X | Leica | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/ |
| ANY-maze | Stoelting Co. | https://www.anymaze.co.uk/index.htm |
| Imaris | Bitplane | https://imaris.oxinst.com/ |
| Patchmaster | HEKA | https://www.heka.com/downloads/downloads_main.html#down_patchmaster |
| Clampfit | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite#gref |
| MiniAnalysis | Synaptosoft | http://www.synaptosoft.com/MiniAnalysis/ |
| FACSDiva software v8 | BD Bioscience | https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software |
| Flow Jo™ v10 | BD Bioscience | https://www.flowjo.com/solutions/flowjo/downloads |